Defining Viral Protein Interactomes Using the Yeast Two-Hybrid Assay

Title: Defining Viral Protein Interactomes Using the Yeast Two-Hybrid Assay

Volume: 2 Issue: 3

Author(s): E. Diefenbach, A. L. Cunningham and R. J. Diefenbach

Affiliation:

Keywords: Yeast two-hybrid assay, interactome, virus, proteomics, drug targets

Abstract: The yeast two-hybrid assay has proved a powerful tool in identifying and characterising binary protein-protein interactions. Not only can it be used to map interacting protein domains, it can also be used to screen cDNA libraries with a desired bait to identify novel binding partners. A number of factors including ease of use, cost effectiveness and suitability for high throughput analysis have made yeast-two hybrid one of the assays of choice for defining protein-protein interaction networks or interactomes for a range of organisms. The focus of this review is on the definition of viral interactomes using the yeast two-hybrid assay and the relevance of such studies to our understanding of viral pathogenesis.

Cite this article as:

Diefenbach E., Cunningham L. A. and Diefenbach J. R., Defining Viral Protein Interactomes Using the Yeast Two-Hybrid Assay, Current Proteomics 2005; 2 (3) . https://dx.doi.org/10.2174/157016405774641138