Abstract
In this review we explore the advantages deriving from the use of either enzymes or binding proteins to develop non-consuming substrate fluorescence biosensors. We report on a novel approach to address the consumption of substrate by enzyme-based biosensors, namely the utilization of apo-enzymes as non-active forms of proteins, which through still able to bind the ligand yet cannot transform it into product. We also report recent studies in which fluorescence labelling of a naturally thermostable binding protein by a fluorescent probe allows a quantitative monitoring of glucose. Finally, we will illustrate a novel methodology based on the utilization of porous silicon chips that allows a nanotechnological approach to the realization of protein arrays for analyses of high medical and biochemical interest.
Keywords: Porous silicon, proteins, diabetes, fluorescence, biosensors
Current Chemical Biology
Title: New Emergent Nanotechnologies in Medical and Biochemical Applications:Advanced Fluorescence Protein-Based Nanosensors
Volume: 1 Issue: 1
Author(s): Maria Staiano, Marcella de Champdore, Stefano Borini, Andrea M. Rossi, Mose Rossi and Sabato D'Auria
Affiliation:
Keywords: Porous silicon, proteins, diabetes, fluorescence, biosensors
Abstract: In this review we explore the advantages deriving from the use of either enzymes or binding proteins to develop non-consuming substrate fluorescence biosensors. We report on a novel approach to address the consumption of substrate by enzyme-based biosensors, namely the utilization of apo-enzymes as non-active forms of proteins, which through still able to bind the ligand yet cannot transform it into product. We also report recent studies in which fluorescence labelling of a naturally thermostable binding protein by a fluorescent probe allows a quantitative monitoring of glucose. Finally, we will illustrate a novel methodology based on the utilization of porous silicon chips that allows a nanotechnological approach to the realization of protein arrays for analyses of high medical and biochemical interest.
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Cite this article as:
Staiano Maria, de Champdore Marcella, Borini Stefano, Rossi M. Andrea, Rossi Mose and D'Auria Sabato, New Emergent Nanotechnologies in Medical and Biochemical Applications:Advanced Fluorescence Protein-Based Nanosensors, Current Chemical Biology 2007; 1 (1) . https://dx.doi.org/10.2174/2212796810701010003
DOI https://dx.doi.org/10.2174/2212796810701010003 |
Print ISSN 2212-7968 |
Publisher Name Bentham Science Publisher |
Online ISSN 1872-3136 |
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