Abstract
Applications of peptide arrays in the field of enzyme profiling are described in detail. Focus is on assay principles for measuring activities of kinases, phosphatases or proteases and on substrate identification/optimization for kinases. Additionally, several examples for reliable identification of substrates for other enzymes like lysine methyl transferases and ATP-ribosyltransferases are given. Finally, use of high-density peptide microarrays for the simultaneous profiling of kinase activities in complex biological fluids like cell lysates or lysates of complete organisms will be analysed. All published examples of peptide arrays used for enzyme profiling are summarized comprehensively.
Keywords: Kinase, peptide microarray, phosphatase, protease, SPOT synthesis, substrate specificity, enzyme profiling, ATP-ribosyltransferases
Mini-Reviews in Organic Chemistry
Title: Profiling of Enzymatic Activities Using Peptide Arrays
Volume: 8 Issue: 2
Author(s): Alexandra Thiele, Stephanie Posel, Michael Spinka, Johannes Zerweck, Ulf Reimer, Ulrich Reineke and Mike Schutkowski
Affiliation:
Keywords: Kinase, peptide microarray, phosphatase, protease, SPOT synthesis, substrate specificity, enzyme profiling, ATP-ribosyltransferases
Abstract: Applications of peptide arrays in the field of enzyme profiling are described in detail. Focus is on assay principles for measuring activities of kinases, phosphatases or proteases and on substrate identification/optimization for kinases. Additionally, several examples for reliable identification of substrates for other enzymes like lysine methyl transferases and ATP-ribosyltransferases are given. Finally, use of high-density peptide microarrays for the simultaneous profiling of kinase activities in complex biological fluids like cell lysates or lysates of complete organisms will be analysed. All published examples of peptide arrays used for enzyme profiling are summarized comprehensively.
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Thiele Alexandra, Posel Stephanie, Spinka Michael, Zerweck Johannes, Reimer Ulf, Reineke Ulrich and Schutkowski Mike, Profiling of Enzymatic Activities Using Peptide Arrays, Mini-Reviews in Organic Chemistry 2011; 8 (2) . https://dx.doi.org/10.2174/157019311795177772
DOI https://dx.doi.org/10.2174/157019311795177772 |
Print ISSN 1570-193X |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6298 |
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