Improved Fmoc Synthesis of Bradykinin

Title: Improved Fmoc Synthesis of Bradykinin

Volume: 18 Issue: 9

Author(s): Rachel J. Stephenson, Paul G. Plieger and David R.K. Harding

Affiliation:

Keywords: Bradykinin, drug delivery, fmoc solid phase peptide synthesis, RP-HPLC, TBTU, DMF, ninhydrin test, IEA, NMR spectroscopy, Fmoc-L-amino acids, TOF, Acetonitrile, EDT, Rink resin, Phenomenexguard columnBradykinin, drug delivery, fmoc solid phase peptide synthesis, RP-HPLC, TBTU, DMF, ninhydrin test, IEA, NMR spectroscopy, Fmoc-L-amino acids, TOF, Acetonitrile, EDT, Rink resin, Phenomenexguard column

Abstract: Two arginine side-chain protecting groups, NG-4-methoxy-2,3,6-trimethylbenzensulfonyl group (Mtr) and NG- 2,2,5,7,8-pentamethylchroman-6-sulfonyl (Pmc), have been investigated at both the Arg1 and/or Arg9 position of the bioactive peptide, Bradykinin using Fluorenylmethyloxycarbonyl (Fmoc) Solid Phase Peptide Synthesis. A more efficient synthesis of the peptide has been found when a combination of Arg(Mtr) is present at position 1 and Arg(Pmc) is present at position 9 giving a cleaved pure yield of 52%.

Cite this article as:

J. Stephenson Rachel, G. Plieger Paul and R.K. Harding David, Improved Fmoc Synthesis of Bradykinin, Protein & Peptide Letters 2011; 18 (9) . https://dx.doi.org/10.2174/092986611796011509