Abstract
The human tyrosinase ectodomain has been expressed in Escherichia coli as a soluble form and purified by immobilized metal affinity column chromatography. The ectodomain exhibited tyrosinase activities toward the hydroxylation and oxidation reactions. Biochemical properties of the ectodomain appeared to be distinct from those of the human tyrosinase, although common features were retained.
Keywords: Biochemical properties, Ectodomain, Expression in Escherichia coli, Human tyrosinase
Protein & Peptide Letters
Title: Characterization of Human Tyrosinase Ectodomain Expressed in Escherichia coli
Volume: 17 Issue: 8
Author(s): Ji-Na Kong, Hee-Jin Lee, Dong-Hyun Jo and Kwang-Hoon Kong
Affiliation:
Keywords: Biochemical properties, Ectodomain, Expression in Escherichia coli, Human tyrosinase
Abstract: The human tyrosinase ectodomain has been expressed in Escherichia coli as a soluble form and purified by immobilized metal affinity column chromatography. The ectodomain exhibited tyrosinase activities toward the hydroxylation and oxidation reactions. Biochemical properties of the ectodomain appeared to be distinct from those of the human tyrosinase, although common features were retained.
Export Options
About this article
Cite this article as:
Kong Ji-Na, Lee Hee-Jin, Jo Dong-Hyun and Kong Kwang-Hoon, Characterization of Human Tyrosinase Ectodomain Expressed in Escherichia coli, Protein & Peptide Letters 2010; 17 (8) . https://dx.doi.org/10.2174/092986610791498957
DOI https://dx.doi.org/10.2174/092986610791498957 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Eriocalyxin B-Induced Apoptosis in Pancreatic Adenocarcinoma Cells Through Thiol-Containing Antioxidant Systems and Downstream Signalling Pathways
Current Molecular Medicine An Evolving Role of Piperazine Moieties in Drug Design and Discovery
Mini-Reviews in Medicinal Chemistry Withdrawal Notice: Comparison of Pharmaceutical Effect of Alemtuzumab and Natalizumab and Their Side Effects in Treatment of Various Stages of Multiple Sclerosis Patients
CNS & Neurological Disorders - Drug Targets Mining the Adenovirus "Virome" for Systemic Oncolytics
Current Pharmaceutical Biotechnology Nanoparticulate Drug Delivery System to Overcome the Limitations of Conventional Curcumin in the Treatment of Various Cancers: A Review
Drug Delivery Letters Exploring the use of Estrogen & Progesterone Replacement Therapy in Subarachnoid Hemorrhage
Current Drug Safety Combined Therapy for Gastrointestinal Carcinomas: Exploiting Synergies Between Gene Therapy and Classical Chemo-Radiotherapy
Current Gene Therapy Lung Cancer Chemotherapy, New Treatment and Related Patents
Recent Patents on Anti-Cancer Drug Discovery Does Religiosity Protect Against Cognitive and Behavioral Decline in Alzheimers Dementia?
Current Alzheimer Research The PI3K Pathway at the Crossroads of Cancer and the Immune System: Strategies for Next Generation Immunotherapy Combinations
Current Cancer Drug Targets Bridging the Gap: The Potential Role of Corticosteroid Binding Globulin in Cardiac Steroid Facilitation
Current Drug Targets MicroRNAs in Arterial Hypertension
Current Topics in Medicinal Chemistry Target-oriented Mechanisms of Novel Herbal Therapeutics in the Chemotherapy of Gastrointestinal Cancer and Inflammation
Current Pharmaceutical Design Kefir: A Synbiotic with Approved Anticarcinogenic Properties
Current Bioactive Compounds 1,2,3-Triazine Scaffold as a Potent Biologically Active Moiety: A Mini Review
Mini-Reviews in Medicinal Chemistry Design, Synthesis, Molecular Docking and Biological Evaluation of 1-(benzo[d]thiazol-2-ylamino)(phenyl)methyl)naphthalen-2-ol Derivatives as Antiproliferative Agents
Letters in Organic Chemistry Characterization of Stem-Like Cells Directly Isolated from Freshly Resected Laryngeal Squamous Cell Carcinoma Specimens
Current Stem Cell Research & Therapy Laminin-332-Integrin Interaction: A Target For Cancer Therapy?
Current Medicinal Chemistry The Discovery and Application of Inhibitors of Glutathione S-Transferase as Therapeutic Agents -A Review
Current Bioactive Compounds Drug-Metabolizing Enzymes Mechanisms and Functions
Current Drug Metabolism