Abstract
Transcriptional repressors and activators are principal control elements in bacterial gene expression. They are involved in the regulation of metabolic pathways, cell division, response to environmental signals, sporulation, replication, to name only a few. Whereas the discovery of these regulators was often fortuitous, a number of molecular, biochemical and biophysical methods have been established that allow to investigate these proteins in great detail and to help understand their functions in the living cell. In this review we focus on a selected set of well characterized transcriptional regulators from Bacillus subtilis and their analysis by methods like EMSA, DNase I footprinting, chemical interference footprinting, in vitro transcription, SELEX, CD measurements, FRET and determination of three-dimensional structure.
Keywords: Transcriptional regulators, CodY, GltC, AhrC, CcpA, CggR, CcpN
Current Protein & Peptide Science
Title: Characterisation of Bacillus subtilis Transcriptional Regulators Involved in Metabolic Processes
Volume: 11 Issue: 4
Author(s): Sabine Brantl and Andreas Licht
Affiliation:
Keywords: Transcriptional regulators, CodY, GltC, AhrC, CcpA, CggR, CcpN
Abstract: Transcriptional repressors and activators are principal control elements in bacterial gene expression. They are involved in the regulation of metabolic pathways, cell division, response to environmental signals, sporulation, replication, to name only a few. Whereas the discovery of these regulators was often fortuitous, a number of molecular, biochemical and biophysical methods have been established that allow to investigate these proteins in great detail and to help understand their functions in the living cell. In this review we focus on a selected set of well characterized transcriptional regulators from Bacillus subtilis and their analysis by methods like EMSA, DNase I footprinting, chemical interference footprinting, in vitro transcription, SELEX, CD measurements, FRET and determination of three-dimensional structure.
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Cite this article as:
Brantl Sabine and Licht Andreas, Characterisation of Bacillus subtilis Transcriptional Regulators Involved in Metabolic Processes, Current Protein & Peptide Science 2010; 11 (4) . https://dx.doi.org/10.2174/138920310791233396
DOI https://dx.doi.org/10.2174/138920310791233396 |
Print ISSN 1389-2037 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5550 |
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