Abstract
The conformation of bovine serum fetuin (BSF) was examined over the pH 7.0-12.9 regions by circular dichroism, intrinsic fluorescence and ANS binding. We observed that at higher pH, BSF exists in alkaline unfolded state. Our results provided evidence that correlates simultaneous formation of secondary structure followed by accumulation of hydrophobic clusters.
Keywords: Acetonitrile, alkali denaturation, bovine serum fetuin, circular dichroism, trifluoroethanol
Protein & Peptide Letters
Title: Stabilization of Folding Intermediate States from Alkaline Induced Unfolded State of Bovine Serum Fetuin in Trifluoroethanol and Acetonitrile
Volume: 17 Issue: 5
Author(s): Basir Ahmad, Zeyaul Islam, Ankita Varshney and Rizwan Hasan Khan
Affiliation:
Keywords: Acetonitrile, alkali denaturation, bovine serum fetuin, circular dichroism, trifluoroethanol
Abstract: The conformation of bovine serum fetuin (BSF) was examined over the pH 7.0-12.9 regions by circular dichroism, intrinsic fluorescence and ANS binding. We observed that at higher pH, BSF exists in alkaline unfolded state. Our results provided evidence that correlates simultaneous formation of secondary structure followed by accumulation of hydrophobic clusters.
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Cite this article as:
Ahmad Basir, Islam Zeyaul, Varshney Ankita and Hasan Khan Rizwan, Stabilization of Folding Intermediate States from Alkaline Induced Unfolded State of Bovine Serum Fetuin in Trifluoroethanol and Acetonitrile, Protein & Peptide Letters 2010; 17 (5) . https://dx.doi.org/10.2174/092986610791112774
DOI https://dx.doi.org/10.2174/092986610791112774 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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