Abstract
A new protease, named SsMTP was identified from the archeon Sulfolobus solfataricus. The enzyme is associated to the cell-membrane and over-produced in response to the peptide-enriched media. SsMTP has a molecular mass of 120 kDa showing optimal activity at pH 2.0 in the temperature range 70 - 90 °C, and a half-life of 20 days at 80 °C. Primary structure analysis revealed that SsMTP represents a novel type of multi-domain thermopsin-like protease containing the catalytic domain followed by two distinct domains, PKD and Y_Y_Y, which are usually involved in a range of protein- protein interactions among the extracellular proteins.
Keywords: Sulfolobus solfataricus, Membrane-bound extracellular protease, Thermopsin-like protease, Multi-domain protease
Protein & Peptide Letters
Title: Identification of a Cell-Bound Extracellular Protease Overproduced by Sulfolobus solfataricus in Peptide-Rich Media
Volume: 17 Issue: 1
Author(s): Raffaele Cannio, Giuliana Catara, Imma Fiume, Marco Balestrieri, Mose Rossi and Gianna Palmieri
Affiliation:
Keywords: Sulfolobus solfataricus, Membrane-bound extracellular protease, Thermopsin-like protease, Multi-domain protease
Abstract: A new protease, named SsMTP was identified from the archeon Sulfolobus solfataricus. The enzyme is associated to the cell-membrane and over-produced in response to the peptide-enriched media. SsMTP has a molecular mass of 120 kDa showing optimal activity at pH 2.0 in the temperature range 70 - 90 °C, and a half-life of 20 days at 80 °C. Primary structure analysis revealed that SsMTP represents a novel type of multi-domain thermopsin-like protease containing the catalytic domain followed by two distinct domains, PKD and Y_Y_Y, which are usually involved in a range of protein- protein interactions among the extracellular proteins.
Export Options
About this article
Cite this article as:
Cannio Raffaele, Catara Giuliana, Fiume Imma, Balestrieri Marco, Rossi Mose and Palmieri Gianna, Identification of a Cell-Bound Extracellular Protease Overproduced by Sulfolobus solfataricus in Peptide-Rich Media, Protein & Peptide Letters 2010; 17 (1) . https://dx.doi.org/10.2174/092986610789909458
DOI https://dx.doi.org/10.2174/092986610789909458 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
PREFACE
Endocrine, Metabolic & Immune Disorders - Drug Targets Interrelationships between Hepatic Fat and Insulin Resistance in Non- Alcoholic Fatty Liver Disease
Current Diabetes Reviews Zinc Complexes Developed as Metallopharmaceutics for Treating Diabetes Mellitus based on the Bio-Medicinal Inorganic Chemistry
Current Topics in Medicinal Chemistry Activated Surfaces for Laser Desorption Mass Spectrometry: Application for Peptide and Protein Analysis
Current Pharmaceutical Design Achieving Better Blood Pressure Control in High-Risk Patients with Type 2 Diabetes Using Combination Antihypertensive Therapy
Vascular Disease Prevention (Discontinued) Effects of Bisphosphonates and Calcium plus Vitamin-D Supplements on Cognitive Function in Postmenopausal Osteoporosis§
Endocrine, Metabolic & Immune Disorders - Drug Targets Abstracts from Speakers
Cardiovascular & Hematological Disorders-Drug Targets Adult Stem Cells and Skeletal Muscle Regeneration
Current Gene Therapy Subject Index to Volume 4
Current Drug Targets - Cardiovascular & Hematological Disorders Tyrosinase Electrochemical Biosensors Monitoring Medicinally Significant Substances
Current Medicinal Chemistry Therapeutic Modulation of Lipoprotein-associated Phospholipase A2 (Lp-PLA2)
Current Pharmaceutical Design Biological Effects of Licochalcones
Mini-Reviews in Medicinal Chemistry Emerging Biologics in the Treatment of Inflammatory Bowel Disease: What is Around the Corner?
Current Drug Targets Controlled Bioactive Molecules Delivery Strategies for Tendon and Ligament Tissue Engineering using Polymeric Nanofibers
Current Pharmaceutical Design Immune Mechanism, Aging, Season and Diseases: Modulatory Role of Melatonin
Immunology, Endocrine & Metabolic Agents in Medicinal Chemistry (Discontinued) Comparison of (+)- and (-)-Hemipalmitoylcarnitinium as Inhibitors of Hepatic Mitochondrial Carnitine Palmitoyltransferases in Diabetic Rats
Medicinal Chemistry Can Dietary Polyphenols Prevent the Formation of Toxic Compounds from Maillard Reaction?
Current Drug Metabolism Mesenchymal Stem Cells: Use in Cartilage Repair
Current Rheumatology Reviews Immunosuppressive and Antiviral Treatment of Inflammatory Cardiomyopathy
Recent Patents on Cardiovascular Drug Discovery Enhanced Butyrylcholinesterase Activity may be the Common Link in Triggering Low-Grade Systemic Inflammation and Decrease in Cognitive Function in Diabetes Mellitus and Alzheimers disease
Current Nutrition & Food Science