Abstract
In the past few years a considerable number of mutagenesis methods and high-throughput screening (HTS) systems have been developed and improved. In parallel, computer programs or software packages for molecular modeling have been further investigated. Thus, the number of examples for successful directed evolution and rational design is increasing constantly. In this review the essential mutagenesis methods and HTS systems, especially for esterases, are described and various examples for the application of these protein engineering tools are provided.
Protein & Peptide Letters
Title: Protein Engineering of Carboxyl Esterases by Rational Design and Directed Evolution
Volume: 16 Issue: 10
Author(s): U.T. Bornscheuer, D. Bottcher and M. Schmidt
Affiliation:
Abstract: In the past few years a considerable number of mutagenesis methods and high-throughput screening (HTS) systems have been developed and improved. In parallel, computer programs or software packages for molecular modeling have been further investigated. Thus, the number of examples for successful directed evolution and rational design is increasing constantly. In this review the essential mutagenesis methods and HTS systems, especially for esterases, are described and various examples for the application of these protein engineering tools are provided.
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Cite this article as:
Bornscheuer U.T., Bottcher D. and Schmidt M., Protein Engineering of Carboxyl Esterases by Rational Design and Directed Evolution, Protein & Peptide Letters 2009; 16 (10) . https://dx.doi.org/10.2174/092986609789071216
DOI https://dx.doi.org/10.2174/092986609789071216 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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