Characterisation of Oxidized Recombinant Human Galectin-1

Title: Characterisation of Oxidized Recombinant Human Galectin-1

Volume: 16 Issue: 10

Author(s): Stacy A. Scott, Andrea Bugarcic and Helen Blanchard

Affiliation:

Keywords: lectin activity, galectin-1 oxidation, protein redox state, Galectin-1

Abstract: Oxidized human galectin-1 plays a role in the immune response to injured axons. Over-expression of galectin-1 by cancer, in combination with cancer associated oxidative stress suggests oxidized human galectin-1 may also play a role(s) in tumourigenesis. Here we generate milligram quantities of oxidized human galectin-1 and undertake biophysical characterization. The protein adopts a number of different states. Two separable oxidized forms are identified that exist as largely mono-disperse solutions at higher milligram/ml concentrations. The presence of disulphide bonds is confirmed for these two protein forms, as is their change in overall shape and loss of lectin activity. Our studies lead to production of a particular mono-disperse oxidized human galectin-1 species that is anticipated most optimal for investigations requiring milligram/ml concentrations such as X-ray crystallography.

Cite this article as:

Scott Stacy A., Bugarcic Andrea and Blanchard Helen, Characterisation of Oxidized Recombinant Human Galectin-1, Protein & Peptide Letters 2009; 16 (10) . https://dx.doi.org/10.2174/092986609789071342