Abstract
Fluorescent live cell imaging has recently been used in numerous studies to examine prions in yeast. These fluorescence studies take advantage of the fact that unlike the normally folded form, the misfolded amyloid form of the prion protein is aggregated. The studies have used fluorescence to identify new prions, to study the transmission of prion from mother to daughter, and to understand the role of molecular chaperones in this transmission. The use of fluorescence imaging complements the more standard methods used to study prion propagation. This review discusses the various studies that have taken advantage of fluorescence imaging technique particularly in regard to understanding the transmission and curing of the [PSI ], the prion form of the translation termination factor Sup35p.
Protein & Peptide Letters
Title: Application of GFP-Labeling to Study Prions in Yeast
Volume: 16 Issue: 6
Author(s): Lois E. Greene, Yang-Nim Park, Daniel C. Masison and Evan Eisenberg
Affiliation:
Abstract: Fluorescent live cell imaging has recently been used in numerous studies to examine prions in yeast. These fluorescence studies take advantage of the fact that unlike the normally folded form, the misfolded amyloid form of the prion protein is aggregated. The studies have used fluorescence to identify new prions, to study the transmission of prion from mother to daughter, and to understand the role of molecular chaperones in this transmission. The use of fluorescence imaging complements the more standard methods used to study prion propagation. This review discusses the various studies that have taken advantage of fluorescence imaging technique particularly in regard to understanding the transmission and curing of the [PSI ], the prion form of the translation termination factor Sup35p.
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Cite this article as:
Greene E. Lois, Park Yang-Nim, Masison C. Daniel and Eisenberg Evan, Application of GFP-Labeling to Study Prions in Yeast, Protein & Peptide Letters 2009; 16 (6) . https://dx.doi.org/10.2174/092986609788490221
DOI https://dx.doi.org/10.2174/092986609788490221 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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