Roles of the Valine Clusters in Domain 3 of the Hemolytic Lectin CEL-III in Its Oligomerization and Hemolytic Abilities

Title: Roles of the Valine Clusters in Domain 3 of the Hemolytic Lectin CEL-III in Its Oligomerization and Hemolytic Abilities

Volume: 16 Issue: 4

Author(s): Keigo Hisamatsu, Hideaki Unno, Shuichiro Goda and Tomomitsu Hatakeyama

Affiliation:

Keywords: Cucumaria echinata, hemolysin, lectin, oligomerization, site-directed mutagenesis

Abstract: The hemolytic lectin CEL-III and its site-directed mutants were expressed in Escherichia coli cells. Replacement of the valine clusters in domain 3 with alanine residues led to increased self-oligomerization in solution and higher hemolytic activity. The results suggest the involvement of these valine clusters in CEL-III oligomerization and hemolytic activity.

Cite this article as:

Hisamatsu Keigo, Unno Hideaki, Goda Shuichiro and Hatakeyama Tomomitsu, Roles of the Valine Clusters in Domain 3 of the Hemolytic Lectin CEL-III in Its Oligomerization and Hemolytic Abilities, Protein & Peptide Letters 2009; 16 (4) . https://dx.doi.org/10.2174/092986609787848054