Abstract
A D-glucose / D-mannose specific lectin from seeds of Canavalia grandiflora (ConGF) was purified by affinity chromatography on Sephadex G-50. By SDS-PAGE ConGF yielded three protein bands with apparent molecular masses of 29-30 kDa (α chain), 16-18 kDa (β fragment) and 12-13 kDa (γ fragment), like other related lectins from the genus Canavalia (Leguminosae). ConGF strongly agglutinates rabbit erythrocytes, has a high content of ASP and SER, and its N-terminal sequence (30 residues) is highly similar to the sequences of other related lectins from subtribe Diocleinae.
Keywords: Canavalia grandiflora, (Leguminosae family), Papilionoideae, N-terminal amino acid, D-glucopyranoside, subtribe Diocleinae
Protein & Peptide Letters
Title: Purification and Partial Characterization of a Lectin from Canavalia Grandiflora Benth. Seeds
Volume: 9 Issue: 1
Author(s): V. M. Ceccatto, B. S. Cavada, E. P. Nunes, N. A.P. Nogueira, M. B. Grangeiro, F.B. M.B. Moreno, E. H. Teixeira, A. H. Sampaio, M. A.O. Alves, M. V. Ramos, J. J. Calvete and T. B. Grangeiro
Affiliation:
Keywords: Canavalia grandiflora, (Leguminosae family), Papilionoideae, N-terminal amino acid, D-glucopyranoside, subtribe Diocleinae
Abstract: A D-glucose / D-mannose specific lectin from seeds of Canavalia grandiflora (ConGF) was purified by affinity chromatography on Sephadex G-50. By SDS-PAGE ConGF yielded three protein bands with apparent molecular masses of 29-30 kDa (α chain), 16-18 kDa (β fragment) and 12-13 kDa (γ fragment), like other related lectins from the genus Canavalia (Leguminosae). ConGF strongly agglutinates rabbit erythrocytes, has a high content of ASP and SER, and its N-terminal sequence (30 residues) is highly similar to the sequences of other related lectins from subtribe Diocleinae.
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Ceccatto M. V., Cavada S. B., Nunes P. E., Nogueira A.P. N., Grangeiro B. M., Moreno M.B. F.B., Teixeira H. E., Sampaio H. A., Alves A.O. M., Ramos V. M., Calvete J. J. and Grangeiro B. T., Purification and Partial Characterization of a Lectin from Canavalia Grandiflora Benth. Seeds, Protein & Peptide Letters 2002; 9 (1) . https://dx.doi.org/10.2174/0929866023409002
DOI https://dx.doi.org/10.2174/0929866023409002 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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