Propionate Sensor Using Coenzyme-A Transferase and Acyl-CoA Oxidase

Title: Propionate Sensor Using Coenzyme-A Transferase and Acyl-CoA Oxidase

Volume: 15 Issue: 8

Author(s): Koji Sode, Wakako Tsugawa, Minoru Aoyagi, Eranna Rajashekhara and Kazuya Watanabe

Affiliation:

Keywords: Propionate, volatile fatty acid, propionate CoA transferase, short-chain acyl-CoA oxidase, amperometry, enzyme sensor

Abstract: We developed an amperometric propionate sensor using comprised of two recombinant enzymes, propionate coenzyme A CoA transferase from Clostridium propionicum and short-chain acyl-CoA oxidase from Arabidopsis thaliana. Response current increased linearly with increase in propionate concentration from 10 μM to 100 μM. The detection limit was 10 μM propionate.

Cite this article as:

Sode Koji, Tsugawa Wakako, Aoyagi Minoru, Rajashekhara Eranna and Watanabe Kazuya, Propionate Sensor Using Coenzyme-A Transferase and Acyl-CoA Oxidase, Protein & Peptide Letters 2008; 15 (8) . https://dx.doi.org/10.2174/092986608785203737