Influence of the N- and C-Terminal Regions of Leu-Lys Rich Antimicrobial Peptide on Antimicrobial Activity

Title: Influence of the N- and C-Terminal Regions of Leu-Lys Rich Antimicrobial Peptide on Antimicrobial Activity

Volume: 15 Issue: 2

Author(s): Yoonkyung Park, Hae Kyun Park, Hak-Tae Lim, Byung Jo Chae, Kyung-Soo Hahm, Hae Kyun Park and Byung Jo Chae

Affiliation:

Keywords: Antimicrobial activity, Leu-Lys rich peptide, P11 peptide, scanning electron microscopy

Abstract: P5 (KWKKLLKKPLLKKLLKKL-NH2) is an antibacterial 18-mer Leu-Lys rich peptide from CA (1-8)-MA (1-12) hybrid peptide (CA-MA). Here we show that decreasing the net hydrophobicity and charge of CA-MA by deleting Leu- or Lys- of the N- or C-terminal regions of P5 (P10 or P11). The antimicrobial activity of the peptides was measured by their growth inhibitory effect upon S. aureus, B. subtilis, P. aeruginosa, S. typhimurium, E. coli, T. beigelii and C. albicans. Antimicrobial activity required a full length C-terminus. Confocal microscopy showed that P11 was located in the plasma membrane. In this study, P11, K3K4L5L6-deleted peptide, acted independent on the ionic environment. Furthermore, P11 causes significant morphological alterations of the fungal surfaces as shown by scanning electron microscopy.

Cite this article as:

Park Yoonkyung, Park Kyun Hae, Lim Hak-Tae, Chae Jo Byung, Hahm Kyung-Soo, Kyun Park Hae and Jo Chae Byung, Influence of the N- and C-Terminal Regions of Leu-Lys Rich Antimicrobial Peptide on Antimicrobial Activity, Protein & Peptide Letters 2008; 15 (2) . https://dx.doi.org/10.2174/092986608783489544