Abstract
To elucidate potential biological roles of human β-synuclein, we studied its conformational changes under various conditions. The structural and functional properties of β-synuclein were characterized using biochemical and biophysical methods including: a functional assay, mass spectrometry, size exclusion chromatography, circular dichroism (CD), and fluorescence spectroscopy. The results showed β-synuclein has a high proportion of random coil in solution.
Keywords: β-Synuclein, circular dichroism, fluorescence, conformational change
Protein & Peptide Letters
Title: Spectroscopic and Functional Characterization of Human β-Synuclein
Volume: 14 Issue: 10
Author(s): Juhyung Lee, Heejin Hwang and T. Doohun Kim
Affiliation:
Keywords: β-Synuclein, circular dichroism, fluorescence, conformational change
Abstract: To elucidate potential biological roles of human β-synuclein, we studied its conformational changes under various conditions. The structural and functional properties of β-synuclein were characterized using biochemical and biophysical methods including: a functional assay, mass spectrometry, size exclusion chromatography, circular dichroism (CD), and fluorescence spectroscopy. The results showed β-synuclein has a high proportion of random coil in solution.
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Cite this article as:
Lee Juhyung, Hwang Heejin and Kim Doohun T., Spectroscopic and Functional Characterization of Human β-Synuclein, Protein & Peptide Letters 2007; 14 (10) . https://dx.doi.org/10.2174/092986607782541132
DOI https://dx.doi.org/10.2174/092986607782541132 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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