Abstract
Mycobacterium Tuberculosis HSP16.3 is a major antigen maximally expressed during the stationary phase. Previous studies showed that HSP16.3 can function as a molecular chaperone in vitro. Here, crystallization trails of HSP 16.3 were reported. A kind of crystal can be diffracted to 2.8 A resolution at the ”Photon Factory“, a synchrotron light source in Japan. The crystal displayed the space group R3 with unit cell parameters a=b=110 A , c=152 A and gamma=120 degree. Assuming the presence of 9 HSP16.3 molecules in an asymmetric unit, it gives a Vm= 0.73 A 3 / Da. and solvent content of 35percent by volume.
Keywords: MYCOBACTERIUM TUBERCULOSIS HSP16.3, IPTG isopropyl ß-D-thiogalacto-pyranoside, MPD 2-Methyl-2,4-Pentanediol, sHSP small heat shock protein, MAD Multiwavelength Anomalous Dispersion, molecular chaperone activity
Protein & Peptide Letters
Title: Preliminary X-Ray Crystallographic Studies Of The Mycobacterium Tuberculosis Hsp16.3 Molecular Chaperone
Volume: 8 Issue: 6
Author(s): Y. Chen, J. An, Y. Ding, H. Dai, Q. Mao, L. Feng, B. Liu, Y. Chang, F. Chen, H. He, H. Tang, Z. Chang and Z. Rao
Affiliation:
Keywords: MYCOBACTERIUM TUBERCULOSIS HSP16.3, IPTG isopropyl ß-D-thiogalacto-pyranoside, MPD 2-Methyl-2,4-Pentanediol, sHSP small heat shock protein, MAD Multiwavelength Anomalous Dispersion, molecular chaperone activity
Abstract: Mycobacterium Tuberculosis HSP16.3 is a major antigen maximally expressed during the stationary phase. Previous studies showed that HSP16.3 can function as a molecular chaperone in vitro. Here, crystallization trails of HSP 16.3 were reported. A kind of crystal can be diffracted to 2.8 A resolution at the ”Photon Factory“, a synchrotron light source in Japan. The crystal displayed the space group R3 with unit cell parameters a=b=110 A , c=152 A and gamma=120 degree. Assuming the presence of 9 HSP16.3 molecules in an asymmetric unit, it gives a Vm= 0.73 A 3 / Da. and solvent content of 35percent by volume.
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Chen Y., An J., Ding Y., Dai H., Mao Q., Feng L., Liu B., Chang Y., Chen F., He H., Tang H., Chang Z. and Rao Z., Preliminary X-Ray Crystallographic Studies Of The Mycobacterium Tuberculosis Hsp16.3 Molecular Chaperone, Protein & Peptide Letters 2001; 8 (6) . https://dx.doi.org/10.2174/0929866013409111
DOI https://dx.doi.org/10.2174/0929866013409111 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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