The Native Gene of Anti-Lps Factor from Tachypleus tridentatus: Cloning, Expression and its Bacteriostatic Activity in Vitro

Dong-Ning      Wang; Lin      Chen; Jie-Wu      Liu; Zhi-Yong      He; Wei-Jie      Zhang; Xiang-Fu      Wu

Abstract

The native gene information of anti-LPS factor from Tachypleus tridentatus was first reported in this study. The total RNA was extracted from amebocytes of Tachypleus tridentatus and the first-strand cDNA was synthesized by RT-PCR. Then the amplified cDNA of TALF was cloned and expressed in Pichia pastoris. The biological activity of the recombinant proteins obtained have been confirmed by LPS neutralization assay and bacteriostatic assay in vitro.

Keywords: ANTI-LPS FACTOR, TACHYPLEUS TRIDENTATUS, CLONING, EXPRESSION, BACTERIOSTATIC ACTIVITY, Lipopolysaccharide (LPS), Endotoxin, Limulus polyphemus, Degenerate Oligonucleotides, LPS neutralization assay, Bacteriostatic assay

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Protein & Peptide Letters

Title: The Native Gene of Anti-Lps Factor from Tachypleus tridentatus: Cloning, Expression and its Bacteriostatic Activity in Vitro

Volume: 8 Issue: 4

Author(s): Dong-Ning Wang, Lin Chen, Jie-Wu Liu, Zhi-Yong He, Wei-Jie Zhang and Xiang-Fu Wu

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Abstract: The native gene information of anti-LPS factor from Tachypleus tridentatus was first reported in this study. The total RNA was extracted from amebocytes of Tachypleus tridentatus and the first-strand cDNA was synthesized by RT-PCR. Then the amplified cDNA of TALF was cloned and expressed in Pichia pastoris. The biological activity of the recombinant proteins obtained have been confirmed by LPS neutralization assay and bacteriostatic assay in vitro.

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Wang Dong-Ning, Chen Lin, Liu Jie-Wu, He Zhi-Yong, Zhang Wei-Jie and Wu Xiang-Fu, The Native Gene of Anti-Lps Factor from Tachypleus tridentatus: Cloning, Expression and its Bacteriostatic Activity in Vitro, Protein & Peptide Letters 2001; 8 (4) . https://dx.doi.org/10.2174/0929866013409337