Abstract
The hydroxyapatite elution behavior of 3 fragments of human XPA (M98-F219 = XPA-MBD M59-F219 = XPA-EM M59-M273 = XPA-deltaN58) and full-length human XPA containing a 6-residue, N-terminal histidine tag (h6-XPA) are described. With increasing concentration of potassium phosphate the proteins elute in the order: XPA-EM, XPA-MBD, XPA-deltaN58, and h6-XPA. If hydroxyapatite affinity is related to DNA-binding affinity, then h6-XPA and XPA-deltaN58 may bind DNA more tightly than XPA-MBD and XPA-EM.
Keywords: REPAIR PROTEIN XPA, Nucleotide excision repair (NER), XPA gene
Protein & Peptide Letters
Title: Hydroxyapatite Elution Behavior of Human Nucleotide Excision Repair Protein XPA and Fragments of XPA
Volume: 8 Issue: 5
Author(s): Garry W. Buchko and Michael A. Kennedy
Affiliation:
Keywords: REPAIR PROTEIN XPA, Nucleotide excision repair (NER), XPA gene
Abstract: The hydroxyapatite elution behavior of 3 fragments of human XPA (M98-F219 = XPA-MBD M59-F219 = XPA-EM M59-M273 = XPA-deltaN58) and full-length human XPA containing a 6-residue, N-terminal histidine tag (h6-XPA) are described. With increasing concentration of potassium phosphate the proteins elute in the order: XPA-EM, XPA-MBD, XPA-deltaN58, and h6-XPA. If hydroxyapatite affinity is related to DNA-binding affinity, then h6-XPA and XPA-deltaN58 may bind DNA more tightly than XPA-MBD and XPA-EM.
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Cite this article as:
Buchko W. Garry and Kennedy A. Michael, Hydroxyapatite Elution Behavior of Human Nucleotide Excision Repair Protein XPA and Fragments of XPA, Protein & Peptide Letters 2001; 8 (5) . https://dx.doi.org/10.2174/0929866013409300
DOI https://dx.doi.org/10.2174/0929866013409300 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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