Abstract
A humanized version of the apoptosis-inducing mouse anti-human Fas monoclonal antibody, HFE7A, is under further development for the treatment of autoimmune diseases such as rheumatoid arthritis. We have crystallized the antigen-binding fragment (Fab) of the humanized HFE7A. The crystals belong to the orthorhombic space group P2 1 2 1 2 1 with cell dimensions a = 54.4 Å, b = 82.7 Å, c = 104.9 Å and contain one Fab molecule in the asymmetric unit. X-ray diffraction data were collected to 2.8 Å resolution.
Keywords: fab fragment, humanized hfe7a, fas monoclonal antibody
Protein & Peptide Letters
Title: Crystallization and Preliminary X-Ray Studies of the Fab Fragment from a Humanized Version of the Mouse Anti-Human Fas Antibody Hfe7a
Volume: 9 Issue: 3
Author(s): Shuichiro Ito, Tomoko Takayama, Hiroyuki Hanzawa, Tohru Takahashi, Kenji Miyadai, Nobufusa Serizawa, Hideyuki Haruyama and Tadashi Hata
Affiliation:
Keywords: fab fragment, humanized hfe7a, fas monoclonal antibody
Abstract: A humanized version of the apoptosis-inducing mouse anti-human Fas monoclonal antibody, HFE7A, is under further development for the treatment of autoimmune diseases such as rheumatoid arthritis. We have crystallized the antigen-binding fragment (Fab) of the humanized HFE7A. The crystals belong to the orthorhombic space group P2 1 2 1 2 1 with cell dimensions a = 54.4 Å, b = 82.7 Å, c = 104.9 Å and contain one Fab molecule in the asymmetric unit. X-ray diffraction data were collected to 2.8 Å resolution.
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Cite this article as:
Ito Shuichiro, Takayama Tomoko, Hanzawa Hiroyuki, Takahashi Tohru, Miyadai Kenji, Serizawa Nobufusa, Haruyama Hideyuki and Hata Tadashi, Crystallization and Preliminary X-Ray Studies of the Fab Fragment from a Humanized Version of the Mouse Anti-Human Fas Antibody Hfe7a, Protein & Peptide Letters 2002; 9 (3) . https://dx.doi.org/10.2174/0929866023408715
DOI https://dx.doi.org/10.2174/0929866023408715 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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