Abstract
The potential function of neuronal tau was found by our recent studies on the effect of tau on the melting temperature of both calf thymus DNA and plasmid pBluescript-II SK (Hua and He, Chin. Sci. Bull. 2000, 45:999-1001). Herein we examined whether or not the interaction of tau with DNA was related to phosphorylation and aggregation. Tau, phosphorylated by neuronal cdc2-like kinase, associated with DNA as shown by electrophoretic mobility shift assay. Similar to native tau, phosphorylated tau could increase the melting temperature of calf thymus DNA. When tau was aggregated or treated with formaldehyde, neither native tau nor phosphorylated tau kept its ability to interact with DNA, suggesting that binding of tau to DNA was in an aggregation-dependent, and a phosphorylation-independent, manner.
Keywords: Phosphorylation, Tau Binding, pBluescript-II, electrophoretic mobility
Protein & Peptide Letters
Title: Effect of Phosphorylation and Aggregation on Tau Binding to Dna
Volume: 9 Issue: 4
Author(s): Qian Hua and Rong-Qiao He
Affiliation:
Keywords: Phosphorylation, Tau Binding, pBluescript-II, electrophoretic mobility
Abstract: The potential function of neuronal tau was found by our recent studies on the effect of tau on the melting temperature of both calf thymus DNA and plasmid pBluescript-II SK (Hua and He, Chin. Sci. Bull. 2000, 45:999-1001). Herein we examined whether or not the interaction of tau with DNA was related to phosphorylation and aggregation. Tau, phosphorylated by neuronal cdc2-like kinase, associated with DNA as shown by electrophoretic mobility shift assay. Similar to native tau, phosphorylated tau could increase the melting temperature of calf thymus DNA. When tau was aggregated or treated with formaldehyde, neither native tau nor phosphorylated tau kept its ability to interact with DNA, suggesting that binding of tau to DNA was in an aggregation-dependent, and a phosphorylation-independent, manner.
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Cite this article as:
Hua Qian and He Rong-Qiao, Effect of Phosphorylation and Aggregation on Tau Binding to Dna, Protein & Peptide Letters 2002; 9 (4) . https://dx.doi.org/10.2174/0929866023408652
DOI https://dx.doi.org/10.2174/0929866023408652 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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