Production of Recombinant Human Calcitonin from Silkworm (B. Mori) Larvae infected by Baculovirus

Title: Production of Recombinant Human Calcitonin from Silkworm (B. Mori) Larvae infected by Baculovirus

Volume: 9 Issue: 4

Author(s): Guan-zhen Yang, Zhen-zhen Chen, Da-fu Cui, Bo-liang Li and Xiang-fu Wu

Affiliation:

Keywords: Human Calcitonin, hmCT-GST fusion, recombinant baculoviruses, silkworm larvae, hypercalcemic effect

Abstract: A synthetic modified gene encoding the human calcitonin analog (hmCT) was expressed by use of the baculovirus expression system. After injection with recombinant baculoviruses, the hmCT-GST fusion protein was produced within the silkworm larvae. The fusion protein was purified by affinity chromatography. Biological activity of hmCT for hypercalcemic effect was determined in normal rats.

Cite this article as:

Yang Guan-zhen, Chen Zhen-zhen, Cui Da-fu, Li Bo-liang and Wu Xiang-fu, Production of Recombinant Human Calcitonin from Silkworm (B. Mori) Larvae infected by Baculovirus, Protein & Peptide Letters 2002; 9 (4) . https://dx.doi.org/10.2174/0929866023408689