Low Content of Protein S29 in Ribosomes of Human Lung Cancer Cell Line A549: Detected by Twodimensional Electrophoresis

Title: Low Content of Protein S29 in Ribosomes of Human Lung Cancer Cell Line A549: Detected by Twodimensional Electrophoresis

Volume: 10 Issue: 1

Author(s): Zhi-Dong Zhou, Lei Bao, Ding-Gan Liu, Min-Qian Li, Yin-Zi Ge, Ying-Ling Huang and Wang-Yi Liu

Affiliation:

Keywords: human liver cancer cell line 7721, human lung cancer cell line a549, human lung normal cell line 2bs, human lung cancer, northern blot, ribosomal protein s29, 2-dimensional polyacrylamide gel electrophoresis, cbb-chromium modified thiosulfate-silver staining

Abstract: This study revealed that the content of protein S29 in ribosomes of cancer cell line A549 was distinctly low (equivalent to about 30% of that of 2BS). The conclusion was acquired based on the ratios of spot volume of ribosomal protein S29 to that of several other ribosomal proteins (S29 / L37a, S29 / L38, S29 / S27 and S29 / S28) in the same gel plate. The possible biological roles of ribosomal protein S29 in malignant transformation and translation regulation are briefly discussed.

Cite this article as:

Zhou Zhi-Dong, Bao Lei, Liu Ding-Gan, Li Min-Qian, Ge Yin-Zi, Huang Ying-Ling and Liu Wang-Yi, Low Content of Protein S29 in Ribosomes of Human Lung Cancer Cell Line A549: Detected by Twodimensional Electrophoresis, Protein & Peptide Letters 2003; 10 (1) . https://dx.doi.org/10.2174/0929866033408273