Abstract
An efficient fusion expression system with a small fusion partner, His6-tagged N-terminal fragment of staphylococcal nuclease R, has been constructed and tested with two genes. The results show that the system is not only suitable for overexpression of small proteins and peptides but simplifies purification of target proteins and peptides. The study also provides a practical method for preparation of isotope-labeled protein sample for NMR analysis.
Keywords: protein expression, small fusion partner, nmr sample preparation
Protein & Peptide Letters
Title: An Efficient Fusion Expression System For Protein And Peptide Overexpression In Escherichia Coli And Nmr Sample Preparation
Volume: 10 Issue: 2
Author(s): Yuan Cheng, Dongsheng Liu, Yanming Feng and Guozhong Jing
Affiliation:
Keywords: protein expression, small fusion partner, nmr sample preparation
Abstract: An efficient fusion expression system with a small fusion partner, His6-tagged N-terminal fragment of staphylococcal nuclease R, has been constructed and tested with two genes. The results show that the system is not only suitable for overexpression of small proteins and peptides but simplifies purification of target proteins and peptides. The study also provides a practical method for preparation of isotope-labeled protein sample for NMR analysis.
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Cite this article as:
Cheng Yuan, Liu Dongsheng, Feng Yanming and Jing Guozhong, An Efficient Fusion Expression System For Protein And Peptide Overexpression In Escherichia Coli And Nmr Sample Preparation, Protein & Peptide Letters 2003; 10 (2) . https://dx.doi.org/10.2174/0929866033479112
DOI https://dx.doi.org/10.2174/0929866033479112 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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