Obtaining Site-Specific Calcium-Binding Affinities Of Calmodulin

Title: Obtaining Site-Specific Calcium-Binding Affinities Of Calmodulin

Volume: 10 Issue: 4

Author(s): Jenny J. Yang, Amy Gawthrop and Yiming Ye

Affiliation:

Keywords: calmodulin, calcium binding proteins, ef-hand calcium binding motifs, metal-binding affinity, cooperativity, cd2, protein engineering

Abstract: Calmodulin (CaM) is an EF-hand Ca(II)-binding protein involved in the regulation of many important biological processes. To date, there is a wealth of information available concerning studies to obtain site-specific calcium binding affinities of CaM, and further to estimate the cooperativity of calcium binding using mutational studies, peptide models, and proteolytic fragmentation. In this paper, we will discuss the energetics of calcium binding and the strong relationship between calcium binding cooperativity and conformational change. We then explain the difficulty of studying key determinants of calcium binding affinity of CaM due to the large change of calcium binding affinity upon mutation. Subsequently, we will introduce “grafting” as a novel approach to obtain the site-specific metal binding properties of calmodulin.

Cite this article as:

Yang J. Jenny, Gawthrop Amy and Ye Yiming, Obtaining Site-Specific Calcium-Binding Affinities Of Calmodulin, Protein & Peptide Letters 2003; 10 (4) . https://dx.doi.org/10.2174/0929866033478852