Abstract
The interaction of activated papain with low molecular weight cystatin (Mr 12500) purified from human placenta has been studied. Analysis of inhibition of caesinolytic activity of papain by cystatin showed stoichiometry of 1:1. Kinetic studies gave an inhibition constant (Ki) value of 5.5 x 10 -8 M and association rate constant (K+1) value of 3.4 x 10 4 (M-1 s-1). All spectroscopic studies showed conformational changes in both papain and cystatin on formation of complex. The data suggest perturbation of environment of aromatic residues and change of their native structure and conformation thereby shedding light on the behaviour of cystatins, especially interaction of placental cystatin with thiol protease inhibitors.
Keywords: cystatin, far uv-cd spectroscopy, fluorescence emission, human placenta, papain, uvabsorption
Protein & Peptide Letters
Title: Studies on the Interaction of Papain with Human Placental Cystatin by Uv, Fluorescence and Cd Spectroscopy
Volume: 11 Issue: 6
Author(s): Fouzia Rashid, S. P. Baba, Sandeep Sharma and B. Bano
Affiliation:
Keywords: cystatin, far uv-cd spectroscopy, fluorescence emission, human placenta, papain, uvabsorption
Abstract: The interaction of activated papain with low molecular weight cystatin (Mr 12500) purified from human placenta has been studied. Analysis of inhibition of caesinolytic activity of papain by cystatin showed stoichiometry of 1:1. Kinetic studies gave an inhibition constant (Ki) value of 5.5 x 10 -8 M and association rate constant (K+1) value of 3.4 x 10 4 (M-1 s-1). All spectroscopic studies showed conformational changes in both papain and cystatin on formation of complex. The data suggest perturbation of environment of aromatic residues and change of their native structure and conformation thereby shedding light on the behaviour of cystatins, especially interaction of placental cystatin with thiol protease inhibitors.
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Cite this article as:
Rashid Fouzia, Baba P. S., Sharma Sandeep and Bano B., Studies on the Interaction of Papain with Human Placental Cystatin by Uv, Fluorescence and Cd Spectroscopy, Protein & Peptide Letters 2004; 11 (6) . https://dx.doi.org/10.2174/0929866043406364
DOI https://dx.doi.org/10.2174/0929866043406364 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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