Abstract
Significant efforts are put into the design of large-scale purification processes of proteins due to great demands regarding cost efficiency and safety. In order to design an effective purification scheme the unit operations need to be reduced to a minimum. In this review we are discussing proteinaceous ligands as well as small synthetic mimics for use in affinity chromatography for large-scale applications. Different advantages as well as drawbacks of the two approaches are outlined.
Keywords: affinity chromatography, cleaning-in-place (cip), deamidation, protein a, protein g, protein engineering, stabilization
Protein & Peptide Letters
Title: Affinity Ligands for Industrial Protein Purification
Volume: 12 Issue: 4
Author(s): Martin Linhult, Susanne Gulich and Sophia Hober
Affiliation:
Keywords: affinity chromatography, cleaning-in-place (cip), deamidation, protein a, protein g, protein engineering, stabilization
Abstract: Significant efforts are put into the design of large-scale purification processes of proteins due to great demands regarding cost efficiency and safety. In order to design an effective purification scheme the unit operations need to be reduced to a minimum. In this review we are discussing proteinaceous ligands as well as small synthetic mimics for use in affinity chromatography for large-scale applications. Different advantages as well as drawbacks of the two approaches are outlined.
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Cite this article as:
Linhult Martin, Gulich Susanne and Hober Sophia, Affinity Ligands for Industrial Protein Purification, Protein & Peptide Letters 2005; 12 (4) . https://dx.doi.org/10.2174/0929866053765662
DOI https://dx.doi.org/10.2174/0929866053765662 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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