Abstract
The technique of gene targeting (knockout) has swept through biomedical research. Cytokine research has been revolutionized by knockouts and since then this technique has been widely utilized in various research fields including immunological, inflammation research and human disease model. This paper focuses on knockout mice in tuberculosis research among many infectious diseases. We have generated several knockout mice for inflammation research. After we infected various kinds of knockout mice suffering from Mycobacterium tuberculosis by aerosol infection, we investigated the roles of cytokines and transcription factors that regulate cytokines. We used knockout mice lacking IFN-γ, TNF-α, IL-18, IL-1 α / β, IL-4, IL-1 type 1 receptor, NF-IL6, TLR-2, TLR-6, interferon regulatory factor-1 (IRF-1), NK-κB p50, signal transducer and activator of transcription (STAT)1, STAT4, NKT cells and MyD88 genes in our experimental tuberculosis research. M. tuberculosis-infected knockout mice displayed various histopathologies depending on the degree of importance of the molecules in defense against tuberculosis. IFN-γ, TNF-α, IRF-1, NF-IL6, NF-κB p50, STAT1 and STAT4 knockout mice succumbed to M. tuberculosis infection over time. The results indicate that these molecules play major roles for defense against tuberculosis. These knockout mice are essential for investigating their roles in experimental tuberculosis.
Keywords: knockout mice, tuberculosis, mycobacterial infection, cytokine, transcription factor
Current Respiratory Medicine Reviews
Title: Pulmonary Tuberculosis in Various Gene Knockout Mice With Special Emphasis on Roles of Cytokines and Transcription Factors
Volume: 1 Issue: 1
Author(s): Isamu Sugawara, Hiroyuki Yamada and Ruiru Shi
Affiliation:
Keywords: knockout mice, tuberculosis, mycobacterial infection, cytokine, transcription factor
Abstract: The technique of gene targeting (knockout) has swept through biomedical research. Cytokine research has been revolutionized by knockouts and since then this technique has been widely utilized in various research fields including immunological, inflammation research and human disease model. This paper focuses on knockout mice in tuberculosis research among many infectious diseases. We have generated several knockout mice for inflammation research. After we infected various kinds of knockout mice suffering from Mycobacterium tuberculosis by aerosol infection, we investigated the roles of cytokines and transcription factors that regulate cytokines. We used knockout mice lacking IFN-γ, TNF-α, IL-18, IL-1 α / β, IL-4, IL-1 type 1 receptor, NF-IL6, TLR-2, TLR-6, interferon regulatory factor-1 (IRF-1), NK-κB p50, signal transducer and activator of transcription (STAT)1, STAT4, NKT cells and MyD88 genes in our experimental tuberculosis research. M. tuberculosis-infected knockout mice displayed various histopathologies depending on the degree of importance of the molecules in defense against tuberculosis. IFN-γ, TNF-α, IRF-1, NF-IL6, NF-κB p50, STAT1 and STAT4 knockout mice succumbed to M. tuberculosis infection over time. The results indicate that these molecules play major roles for defense against tuberculosis. These knockout mice are essential for investigating their roles in experimental tuberculosis.
Export Options
About this article
Cite this article as:
Sugawara Isamu, Yamada Hiroyuki and Shi Ruiru, Pulmonary Tuberculosis in Various Gene Knockout Mice With Special Emphasis on Roles of Cytokines and Transcription Factors, Current Respiratory Medicine Reviews 2005; 1 (1) . https://dx.doi.org/10.2174/1573398052953596
DOI https://dx.doi.org/10.2174/1573398052953596 |
Print ISSN 1573-398X |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6387 |
Call for Papers in Thematic Issues
Exposure to PM2.5 components is associated with respiratory diseases
Through continuous research on the relationship between risk factors and health, it has been found that air pollution, especially atmospheric particulate matter pollution, has become one of the main sources of global disease burden. From 1990 to 2022, the concentration of atmospheric particulate matter pollution has increased by more than ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Novel Pthalazinyl Derivatives: Synthesis, Antimycobacterial Activities, and Inhibition of Mycobacterium Tuberculosis Isocitrate Lyase Enzyme
Medicinal Chemistry Tartrate-Resistant Acid Phosphatase: A Target for Anti-Osteoporotic Chemotherapeutics
Current Enzyme Inhibition Development of Neurochemical Normalization (“Agonist Substitution”) Therapeutics for Stimulant Abuse: Focus on the Dopamine Uptake Inhibitor, GBR12909
Current Medicinal Chemistry - Central Nervous System Agents Alternatives to Conventional Vaccines - Mediators of Innate Immunity
Current Drug Targets CD44 and its Role in Inflammation and Inflammatory Diseases
Inflammation & Allergy - Drug Targets (Discontinued) Recent Developments in the Electrochemistry of Some Nitro Compounds of Biological Significance
Current Organic Chemistry Prevention and Therapeutic Strategies of Thromboembolic Events in Patients with Inflammatory Bowel Diseases: A Report of Three Cases
Current Drug Targets Evaluation of Stationary Phases Made by Hydrosilation of Alkynes on Silica Hydride
Current Chromatography Prospects for Developing New Antibacterials Targeting Bacterial Type IIA Topoisomerases
Current Topics in Medicinal Chemistry Current Status and Future Prospects of Small–molecule Protein–protein Interaction (PPI) Inhibitors of Tumor Necrosis Factor (TNF) and Receptor Activator of NF-κB Ligand (RANKL)
Current Topics in Medicinal Chemistry Tuberculosis, BCG Vaccination, and COVID-19: Are They Connected?
Mini-Reviews in Medicinal Chemistry Research Progress in Live Attenuated Brucella Vaccine Development
Current Pharmaceutical Biotechnology New Perspectives on Machine Learning in Drug Discovery
Current Medicinal Chemistry Preface
Current Drug Targets - Infectious Disorders Intraocular Immune Mechanisms in Uveitis
Current Immunology Reviews (Discontinued) Radiopharmaceutical: Revolutionary Agents for Diagnosis
Current Radiopharmaceuticals Meet Our Editorial Board Member
Current Medicinal Chemistry New Antimicrobial Frontiers
Mini-Reviews in Medicinal Chemistry Chemical Constituents and Biological Activities of Simira Genus: A Contribution to the Chemotaxonomic of Rubiaceae Family
The Natural Products Journal Probiotics/Prebiotics in Viral Respiratory Infections: Implication for Emerging Pathogens
Recent Patents on Biotechnology