Two Novel Lectins from Parkia biglandulosa and Parkia roxburghii: Isolation, Physicochemical Characterization, Mitogenicity and Anti- Proliferative Activity

Title: Two Novel Lectins from Parkia biglandulosa and Parkia roxburghii: Isolation, Physicochemical Characterization, Mitogenicity and Anti- Proliferative Activity

Volume: 12 Issue: 6

Author(s): Navjot Kaur, Jatinder Singh, Sukhdev S. Kamboj, Javed N. Agrewala and Manpreet Kaur

Affiliation:

Keywords: anti-proliferative, cell lines, lectin, leguminosae, mannose, mitogenic, parkia

Abstract: Two mannose/glucose specific seed lectins were isolated from Parkia biglandulosa and Parkia roxburghii and were characterized w.r.t various physicochemical properties. Unlike other Parkia lectins a comparison of native and subunit molecular mass showed that both Parkia lectins were heterotetramers. Parkia biglandulosa lectin was found to be T-cell mitogen as revealed by IL-2 bioassay. These lectins showed anti-proliferative effect on two murine macrophage cancer cell lines i.e. P 388DI (50%) and J774 (70%). In addition Parkia roxburghii also inhibited proliferation of HB98 (65.47%), a B-cell hybridoma cell line.

Cite this article as:

Kaur Navjot, Singh Jatinder, Kamboj S. Sukhdev, Agrewala N. Javed and Kaur Manpreet, Two Novel Lectins from Parkia biglandulosa and Parkia roxburghii: Isolation, Physicochemical Characterization, Mitogenicity and Anti- Proliferative Activity, Protein & Peptide Letters 2005; 12 (6) . https://dx.doi.org/10.2174/0929866054395725