Expressed Protein Ligation to Study Protein Interactions: Semi-Synthesis of the G-Protein Alpha Subunit

Lori   L.   Anderson; Garland   R.   Marshall; Thomas   J.   Baranski

Abstract

Interactions between G proteins and GPCRs are fundamental for transmitting signals for a multitude of physiologic responses. Little is known regarding the protein-protein interface between the G protein and the receptor, much less the mechanisms for receptor activation of G proteins. Here, we will describe how expressed protein ligation will aid in the study of protein-protein interactions between semi-synthetic G alpha subunits and GPCRs.

Keywords: g protein, intein, expressed protein ligation, rhodopsin, transducin

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Protein & Peptide Letters

Title: Expressed Protein Ligation to Study Protein Interactions: Semi-Synthesis of the G-Protein Alpha Subunit

Volume: 12 Issue: 8

Author(s): Lori L. Anderson, Garland R. Marshall and Thomas J. Baranski

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Keywords: g protein, intein, expressed protein ligation, rhodopsin, transducin

Abstract: Interactions between G proteins and GPCRs are fundamental for transmitting signals for a multitude of physiologic responses. Little is known regarding the protein-protein interface between the G protein and the receptor, much less the mechanisms for receptor activation of G proteins. Here, we will describe how expressed protein ligation will aid in the study of protein-protein interactions between semi-synthetic G alpha subunits and GPCRs.

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Anderson L. Lori, Marshall R. Garland and Baranski J. Thomas, Expressed Protein Ligation to Study Protein Interactions: Semi-Synthesis of the G-Protein Alpha Subunit, Protein & Peptide Letters 2005; 12 (8) . https://dx.doi.org/10.2174/0929866054864175