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Protein & Peptide Letters

Editor-in-Chief

ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Oligomerisation of Sarcoplasmic Reticulum Ca2+-ATPase Monomers from Skeletal Muscle

Author(s): Daniela Schreiber and Kay Ohlendieck

Volume 14, Issue 3, 2007

Page: [219 - 226] Pages: 8

DOI: 10.2174/092986607780090838

Price: $65

Abstract

The fast-twitch SERCA1 isoform of the sarcoplasmic reticulum Ca2+-ATPase was purified to homogeneity and conjugated to peroxidase. The SERCA1 probe showed high affinity binding to the immobilized monomeric enzyme, but not crosslinker-stabilized oligomers. This suggests a preferential complex formation via homo-dimerization, rather than interactions with established oligomeric structures.

Keywords: Blot overlay, Ca2+-ATPase, Ca2+-uptake, oligomerisation, sarcoplasmic reticulum, SERCA


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