Abstract
We have previously isolated a cowpea seed defensin with potent antifungal activity. Here we report the cloning of its cDNA obtained from RT-PCR. The sequence of the deduced protein VUDEF exhibited homology to plant defensins. Northern analysis revealed that VUDEF mRNA is accumulated during cowpea seed development and its level increases in seedling tissues after exposure to fungal pathogen and cold.
Keywords: Antifungal peptide, Fusarium oxysporum f. sp. phaseolus, Vigna unguiculata, seedling, phylogenetic relationship
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