Abstract
Recent advances in high throughput screening technologies have accelerated the identification and characterization of potential factors involved in host-virus interactions, facilitating early detection and diagnosis of diseases, as well as providing promising drug targets. The last decade has seen a plethora of successful examples of high throughput screening approaches, especially siRNA screening. With support from protein interaction studies, mRNA expression profiling, and bioinformatics, siRNA screening has also been successfully utilized to identify host factors required for a number of viruses including HIV, West Nile virus and H1N1 virus. Such studies have raised the awareness of virologists, and have opened a new chapter of global analysis of host-pathogen interactions. However, to play a more defining role in prognostics, diagnostics and therapeutics for virus diseases, acknowledged drawbacks, including false positives and negatives, inherent in this technology, must be successfully addressed.
Keywords: Drug target, host factor, pathogen, siRNA, virus, Mycobacterium tuberculosis, high throughput screening, prognostics, hepatitis B virus, infection, hepatocellular carcinoma, replication, genome, viral life cycle, expression, host proteins
Combinatorial Chemistry & High Throughput Screening
Title: The Application of High Throughput siRNA Screening Technology to Study Host-Pathogen Interactions
Volume: 15 Issue: 4
Author(s): Li Ou, Dan Duan, Jinhua Wu, Edouard Nice and Canhua Huang
Affiliation:
Keywords: Drug target, host factor, pathogen, siRNA, virus, Mycobacterium tuberculosis, high throughput screening, prognostics, hepatitis B virus, infection, hepatocellular carcinoma, replication, genome, viral life cycle, expression, host proteins
Abstract: Recent advances in high throughput screening technologies have accelerated the identification and characterization of potential factors involved in host-virus interactions, facilitating early detection and diagnosis of diseases, as well as providing promising drug targets. The last decade has seen a plethora of successful examples of high throughput screening approaches, especially siRNA screening. With support from protein interaction studies, mRNA expression profiling, and bioinformatics, siRNA screening has also been successfully utilized to identify host factors required for a number of viruses including HIV, West Nile virus and H1N1 virus. Such studies have raised the awareness of virologists, and have opened a new chapter of global analysis of host-pathogen interactions. However, to play a more defining role in prognostics, diagnostics and therapeutics for virus diseases, acknowledged drawbacks, including false positives and negatives, inherent in this technology, must be successfully addressed.
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Cite this article as:
Ou Li, Duan Dan, Wu Jinhua, Nice Edouard and Huang Canhua, The Application of High Throughput siRNA Screening Technology to Study Host-Pathogen Interactions, Combinatorial Chemistry & High Throughput Screening 2012; 15 (4) . https://dx.doi.org/10.2174/138620712799361834
DOI https://dx.doi.org/10.2174/138620712799361834 |
Print ISSN 1386-2073 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5402 |
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