Abstract
Fluorescent proteins have revolutionized in vivo biology. Due to their intrinsic brightness, multiple colors, and ease of genetic manipulation, fluorescent proteins have been demonstrated to be the reporters of choice for in vivo imaging. The present report reviews applications of fluorescent proteins for imaging cancer progression, gene expression, angiogenesis, stem cells, bacterial infection, Leishmania infection, and asthma, at the cellular and subcellular level in live mice. With fluorescent-protein-expressing cells and a highly sensitive small animal imaging system, cellular and subcellular dynamics can now be observed in live mice in real time. Such imaging possibilities can provide new visual targets for novel drug therapy. Fluorescent proteins thus enable both micro as well as macro imaging technology and thereby provide the basis for the new field of in vivo cell biology.
Keywords: Asthma, cancer, cellular dynamics, fluorescence imaging, GFP, infection, live mice, metastatic trafficking, nuclear dynamics, RFP, fluorescent-protein-expressing cells, visual drug targets, Fluorescent proteins, stem cells, dual-color cells
Current Pharmaceutical Biotechnology
Title: Cellular and Subcellular Imaging in Live Mice Using Fluorescent Proteins
Volume: 13 Issue: 4
Author(s): Robert M. Hoffman
Affiliation:
Keywords: Asthma, cancer, cellular dynamics, fluorescence imaging, GFP, infection, live mice, metastatic trafficking, nuclear dynamics, RFP, fluorescent-protein-expressing cells, visual drug targets, Fluorescent proteins, stem cells, dual-color cells
Abstract: Fluorescent proteins have revolutionized in vivo biology. Due to their intrinsic brightness, multiple colors, and ease of genetic manipulation, fluorescent proteins have been demonstrated to be the reporters of choice for in vivo imaging. The present report reviews applications of fluorescent proteins for imaging cancer progression, gene expression, angiogenesis, stem cells, bacterial infection, Leishmania infection, and asthma, at the cellular and subcellular level in live mice. With fluorescent-protein-expressing cells and a highly sensitive small animal imaging system, cellular and subcellular dynamics can now be observed in live mice in real time. Such imaging possibilities can provide new visual targets for novel drug therapy. Fluorescent proteins thus enable both micro as well as macro imaging technology and thereby provide the basis for the new field of in vivo cell biology.
Export Options
About this article
Cite this article as:
M. Hoffman Robert, Cellular and Subcellular Imaging in Live Mice Using Fluorescent Proteins, Current Pharmaceutical Biotechnology 2012; 13 (4) . https://dx.doi.org/10.2174/138920112799436311
DOI https://dx.doi.org/10.2174/138920112799436311 |
Print ISSN 1389-2010 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4316 |
Call for Papers in Thematic Issues
Artificial Intelligence in Bioinformatics
Bioinformatics is an interdisciplinary field that analyzes and explores biological data. This field combines biology and information system. Artificial Intelligence (AI) has attracted great attention as it tries to replicate human intelligence. It has become common technology for analyzing and solving complex data and problems and encompasses sub-fields of machine ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Reduced Lipocalin 2 Expression Contributes to Vincristine Resistance in Human Colon Cancer Cells
Recent Patents on Anti-Cancer Drug Discovery Involvement of Orbital Structures in Rheumatic Disease
Current Rheumatology Reviews Viruses and Multiple Sclerosis
CNS & Neurological Disorders - Drug Targets Recognition of Nucleic Acids by Toll-Like Receptors and Development of Immunomodulatory Drugs
Current Medicinal Chemistry New Treatments for COPD in the Elderly
Current Pharmaceutical Design The Impact of Nanobiotechnology on the Development of New Drug Delivery Systems
Current Pharmaceutical Biotechnology Cardiovascular Effects of EGFR (Epidermal Growth Factor Receptor) Monoclonal Antibodies
Cardiovascular & Hematological Agents in Medicinal Chemistry Importance of Fibroblasts-Myofibroblasts in Asthma-Induced Airway Remodeling
Recent Patents on Inflammation & Allergy Drug Discovery An Updated Meta-Analysis of the Efficacy and Safety of Acupuncture Treatment for Vascular Cognitive Impairment Without Dementia
Current Neurovascular Research Advances in Titanium-Catalyzed Synthesis of Chiral Sulfoxide Drugs
Mini-Reviews in Organic Chemistry Developmental Studies of Curcumin NLCs as Safe Alternative in Management of Infectious Childhood Dermatitis
Nanoscience & Nanotechnology-Asia Systems Biology and Computer-Aided Drug Discovery
Current Computer-Aided Drug Design Copper as a Biocidal Tool
Current Medicinal Chemistry Anti-inflammatory and Hypolipidemic Effect of Novel Conjugates with Trolox and Other Antioxidant Acids
Medicinal Chemistry Substrate Specificity of Rat DESC4, a Type II Transmembrane Serine Protease
Protein & Peptide Letters Production and Degradation of Oxygen Metabolites During Inflammatory States in the Human Lung
Current Drug Targets - Inflammation & Allergy Treatment Failure in Ventilator Associated Pneumonia
Current Respiratory Medicine Reviews Toxicological Effects of Dietary Biogenic Amines
Current Nutrition & Food Science Recent Patents for the Treatment of Asthma
Recent Patents on Inflammation & Allergy Drug Discovery Novel Formulations for Oral Allergen Vaccination
Recent Patents on Inflammation & Allergy Drug Discovery