Abstract
Lipid rafts are sphingolipid- and cholesterol-enriched membrane microdomains, which are involved in entry, assembly and budding of various types of viruses. Identification of rafts associated proteins modified by virus infection could therefore provide novel insights into the mechanisms of virus infection as well as the development of new biomarkers for diagnosis and drug development. Proteomic approaches, such as LC-ESI-MS/MS, two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), two-dimensional difference gel electrophoresis (2D-DIGE), isotopecoded affinity tags (ICAT), Proteolytic18O Labeling, isotope Tags for Relative and Absolute Quantification (iTRAQ), Stable Isotope Labeling with Amino acids in Cell culture (SILAC) and Multidimensional Protein Identification Technology (MudPIT), provide the large scale and unbiased platform to determine the dynamic profiles of the lipid rafts proteome. In this review, we summarized the research advance regarding modern proteomics analysis of host lipid rafts alterations in response to diverse virus infection.
Keywords: Lipid rafts, proteomics, virus, microdomains, viruses, infection, biomarkers, 2D-PAGE, ICAT, SILAC, Itraq, sucrose density gradients, heterogeneous, biological processes, signal transduction, cytoskeleton, transcytosis, cholesterol chelating drugs, host cell, receptor molecules, target cell membrane, membrane fusion, human polyomavirus, endocytosis, cholesterol
Combinatorial Chemistry & High Throughput Screening
Title: Proteomics Annotation of Lipid Rafts Modified by Virus Infection
Volume: 15 Issue: 3
Author(s): Na Xie, Kai Huang, Yunlong Lei, Yuping Ran and Canhua Huang
Affiliation:
Keywords: Lipid rafts, proteomics, virus, microdomains, viruses, infection, biomarkers, 2D-PAGE, ICAT, SILAC, Itraq, sucrose density gradients, heterogeneous, biological processes, signal transduction, cytoskeleton, transcytosis, cholesterol chelating drugs, host cell, receptor molecules, target cell membrane, membrane fusion, human polyomavirus, endocytosis, cholesterol
Abstract: Lipid rafts are sphingolipid- and cholesterol-enriched membrane microdomains, which are involved in entry, assembly and budding of various types of viruses. Identification of rafts associated proteins modified by virus infection could therefore provide novel insights into the mechanisms of virus infection as well as the development of new biomarkers for diagnosis and drug development. Proteomic approaches, such as LC-ESI-MS/MS, two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), two-dimensional difference gel electrophoresis (2D-DIGE), isotopecoded affinity tags (ICAT), Proteolytic18O Labeling, isotope Tags for Relative and Absolute Quantification (iTRAQ), Stable Isotope Labeling with Amino acids in Cell culture (SILAC) and Multidimensional Protein Identification Technology (MudPIT), provide the large scale and unbiased platform to determine the dynamic profiles of the lipid rafts proteome. In this review, we summarized the research advance regarding modern proteomics analysis of host lipid rafts alterations in response to diverse virus infection.
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Cite this article as:
Xie Na, Huang Kai, Lei Yunlong, Ran Yuping and Huang Canhua, Proteomics Annotation of Lipid Rafts Modified by Virus Infection, Combinatorial Chemistry & High Throughput Screening 2012; 15 (3) . https://dx.doi.org/10.2174/138620712799218617
DOI https://dx.doi.org/10.2174/138620712799218617 |
Print ISSN 1386-2073 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5402 |
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