Abstract
The use of biodegradable synthetic polyesters as peripheral nerve conduits and the “contact guidance” effect of nano-sized fibrous matrices on neurite outgrowth have been reported; however, no literature exists regarding how these matrices regulate neurite growth-related gene expression. Such information would be useful in the evaluation of the biological applicability of various materials. In this study, two biodegradable synthetic polyesters (PLLA and PBSA) were employed to fabricate a series of micro/nano-fibrous membranes that were used as culture matrices for the in vitro growth of PC12 cells in response to nerve growth factor (NGF). Cell adhesion, proliferation, and differentiation were analyzed in addition to neurite outgrowth- and apoptosis-related gene expression. Our results show that cells grown on electrospun PBSA membranes proliferate to a greater extent than cells cultured on tissue culture polystyrene (TCPS) and other prepared membranes. Compared to TCPS and PBSA, growth on an electrospun PLLA membrane triggers higher GAP43 and MAP2 gene expression in PC12 cells. No significant effect of membrane type on apoptosis-related gene expression was observed. These results indicate that electrospun nano-fibrous PLLA mats are more suitable for use as nerve conduit matrices than conventional non/micro-fibrous PLLA or PBSA membranes.
Keywords: PLLA, PBSA, electrospinning, PC12 cells, neurite outgrowth, Gene Expression, Behavior Analysis, Nano-fibrous Membranes
Call for Papers in Thematic Issues
Role of nanomaterials in fabrication of coatings, Machining and Joining
The application of nanoscience has brought about a revolution in the field of mechanical engineering by providing novel materials, boosting manufacturing processes, and generating cutting-edge products. The purpose of this special issue is to investigate the enormous impact that nanoscience has had on mechanical engineering, with a particular emphasis on ...read more
Advanced Inorganic Nanocomposites and Their Emerging Applications
This special issue collection will highlight developments on the recent trends about the synthesis of metal oxides, nanoclusters, biomaterials, 2D nanomaterials, nanocrystals, nanocomposites, etc. and their applications in electrochemical systems, tissue regeneration, energy storage and harvesting, sensors, etc. The novelty of the methods in the chemical synthesis and their characterizations, ...read more
Applicability of Nanotechnology for Performance Enhancement of Clean Energy Technologies
Population growth, industrialization, and improvement in living quality would lead to further increase in energy demand in near future. Regarding the disadvantages of fossil fuels such as fluctuations in their price, high emissions of greenhouse gases and restriction of their sources, it is crucial to use and exploit alternative energy ...read more
Graphene and 2D Materials for Energy Storage and Conversion
This thematic issue will discuss the recent advances in graphene-based nanomaterials for different energy technologies. Graphene possesses a high surface area, and stable structure and exhibits many interesting electronic, optical, and mechanical properties due to its 2D crystal structure. Graphene is of both fundamental interest and suitable for a wide ...read more
Related Books
Nanoscale Field Effect Transistors: Emerging Applications
Nanoelectronics Devices: Design, Materials, and Applications Part II
Nanoelectronics Devices: Design, Materials, and Applications (Part I)
Role of Nanotechnology in Cancer Therapy
Synthesis and Applications of Semiconductor Nanostructures
Pathways to Green Nanomaterials: Plants as Raw Materials, Reducing Agents and Hosts
Applications of Nanomaterials in Medical Procedures and Treatments
Synthesis of Nanomaterials
Nanobiotechnology: Principles and Applications
Bio-Inspired Nanotechnology
2