Abstract
The Sleeping Beauty (SB) transposase system for somatic integration offers great potential for in vivo gene therapeutic applications and genome engineering. Until recently, however, efficacy of SB transposase as a gene transfer vector especially in large animals was lacking. Herein, we report about the newest viral vector development for delivery of the SB transposase system into large mammals. Over the past decade various hyperactive versions of SB transposase and advanced adenovirus vectors enabling efficient and safe delivery of transgenes in vivo were developed. Already several years ago it was demonstrated that adenovirus vectors can be used for delivery of the SB transposase system into murine liver. Our newest study showed for the first time that a hyperactive transposase system delivered by high-capacity adenoviral vectors can result in somatic integration of exogenous DNA in canine liver, facilitating stabilized transgene expression and phenotypic correction for up to three years in a canine model of human disease. In this review we discuss safety issues and further improvements of this adenovirus based hybrid vector system for somatic integration. In the future this approach paves new paths towards the possible cure of human genetic diseases and novel strategies for in vivo genome engineering in large mammals.
Keywords: Adenovirus, Sleeping Beauty transposase, hybrid vector, persistence, somatic integration, large animal, canine, in vivo, phenotype, cellular genes
Current Gene Therapy
Title: Development of Adenovirus Hybrid Vectors for Sleeping Beauty Transposition in Large Mammals
Volume: 11 Issue: 5
Author(s): Martin Hausl, Wenli Zhang, Richard Voigtlander, Nadine Muther, Christina Rauschhuber and Anja Ehrhardt
Affiliation:
Keywords: Adenovirus, Sleeping Beauty transposase, hybrid vector, persistence, somatic integration, large animal, canine, in vivo, phenotype, cellular genes
Abstract: The Sleeping Beauty (SB) transposase system for somatic integration offers great potential for in vivo gene therapeutic applications and genome engineering. Until recently, however, efficacy of SB transposase as a gene transfer vector especially in large animals was lacking. Herein, we report about the newest viral vector development for delivery of the SB transposase system into large mammals. Over the past decade various hyperactive versions of SB transposase and advanced adenovirus vectors enabling efficient and safe delivery of transgenes in vivo were developed. Already several years ago it was demonstrated that adenovirus vectors can be used for delivery of the SB transposase system into murine liver. Our newest study showed for the first time that a hyperactive transposase system delivered by high-capacity adenoviral vectors can result in somatic integration of exogenous DNA in canine liver, facilitating stabilized transgene expression and phenotypic correction for up to three years in a canine model of human disease. In this review we discuss safety issues and further improvements of this adenovirus based hybrid vector system for somatic integration. In the future this approach paves new paths towards the possible cure of human genetic diseases and novel strategies for in vivo genome engineering in large mammals.
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Cite this article as:
Hausl Martin, Zhang Wenli, Voigtlander Richard, Muther Nadine, Rauschhuber Christina and Ehrhardt Anja, Development of Adenovirus Hybrid Vectors for Sleeping Beauty Transposition in Large Mammals, Current Gene Therapy 2011; 11 (5) . https://dx.doi.org/10.2174/156652311797415890
DOI https://dx.doi.org/10.2174/156652311797415890 |
Print ISSN 1566-5232 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5631 |
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