Fabricating Gradient Hydrogel Scaffolds for 3D Cell Culture

Title: Fabricating Gradient Hydrogel Scaffolds for 3D Cell Culture

Volume: 14 Issue: 4

Author(s): Kaushik Chatterjee, Marian F. Young and Carl G. Simon

Affiliation:

Keywords: Combinatorial methods, high-throughput screening, hydrogels, elastic modulus, gradients, osteoblast, tissue engineering, 3D Cell Culture, drug discovery, embryonic stem cells, Cell Encapsulation

Abstract: Optimizing cell-material interactions is critical for maximizing regeneration in tissue engineering. Combinatorial and high-throughput (CHT) methods can be used to systematically screen tissue scaffolds to identify optimal biomaterial properties. Previous CHT platforms in tissue engineering have involved a two-dimensional (2D) cell culture format where cells were cultured on material surfaces. However, these platforms are inadequate to predict cellular response in a three-dimensional (3D) tissue scaffold. We have developed a simple CHT platform to screen cell-material interactions in 3D culture format that can be applied to screen hydrogel scaffolds. Herein we provide detailed instructions on a method to prepare gradients in elastic modulus of photopolymerizable hydrogels.

Cite this article as:

Chatterjee Kaushik, F. Young Marian and G. Simon Carl, Fabricating Gradient Hydrogel Scaffolds for 3D Cell Culture, Combinatorial Chemistry & High Throughput Screening 2011; 14 (4) . https://dx.doi.org/10.2174/138620711795222455