Abstract
Homologous recombination is almost the only way to modify the genome in a predetermined fashion, despite its quite low frequency in mammalian cells. It has been already reported that the frequency of this biological process can be notably increased by inducing a double strand break (DSB) at target site. This article presents completely chemistrybased artificial restriction DNA cutter (ARCUT) for the promotion of homologous recombination in human cells. This cutter is composed of Ce(IV)/EDTA complex (molecular scissors) and two strands of peptide nucleic acid (PNA), and contains no proteins. Its scission site in the genome is determined simply by Watson-Crick rule so that ARCUT for desired homologous recombination is easily and straightforwardly designed and synthesized. The site-specificity of the scission is high enough to cut human genome at one target site. The DSB induced by this cutter is satisfactorily recognized by the repair system in human cells and promotes the targeted homologous recombination.
Keywords: Genome manipulation, artificial restriction DNA cutter, cerium, peptide nucleic acid, homologous recombination, double strand break, phosphodiester, thymine, pcPNA strands, hypermethylation, X syndrome, fluorescent protein, green fluorescence, adenovirus vector
Current Gene Therapy
Title: Artificial Restriction DNA Cutters to Promote Homologous Recombination in Human Cells
Volume: 11 Issue: 1
Author(s): Hitoshi Katada and Makoto Komiyama
Affiliation:
Keywords: Genome manipulation, artificial restriction DNA cutter, cerium, peptide nucleic acid, homologous recombination, double strand break, phosphodiester, thymine, pcPNA strands, hypermethylation, X syndrome, fluorescent protein, green fluorescence, adenovirus vector
Abstract: Homologous recombination is almost the only way to modify the genome in a predetermined fashion, despite its quite low frequency in mammalian cells. It has been already reported that the frequency of this biological process can be notably increased by inducing a double strand break (DSB) at target site. This article presents completely chemistrybased artificial restriction DNA cutter (ARCUT) for the promotion of homologous recombination in human cells. This cutter is composed of Ce(IV)/EDTA complex (molecular scissors) and two strands of peptide nucleic acid (PNA), and contains no proteins. Its scission site in the genome is determined simply by Watson-Crick rule so that ARCUT for desired homologous recombination is easily and straightforwardly designed and synthesized. The site-specificity of the scission is high enough to cut human genome at one target site. The DSB induced by this cutter is satisfactorily recognized by the repair system in human cells and promotes the targeted homologous recombination.
Export Options
About this article
Cite this article as:
Katada Hitoshi and Komiyama Makoto, Artificial Restriction DNA Cutters to Promote Homologous Recombination in Human Cells, Current Gene Therapy 2011; 11 (1) . https://dx.doi.org/10.2174/156652311794520094
DOI https://dx.doi.org/10.2174/156652311794520094 |
Print ISSN 1566-5232 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5631 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Pleural Involvement in Systemic Disorders
Current Drug Targets - Inflammation & Allergy Targeting IAPs as An Approach to Anti-Cancer Therapy
Current Topics in Medicinal Chemistry Neurotrophic Factors as a Protective Strategy in Parkinsons Disease
CNS & Neurological Disorders - Drug Targets First Phase Insulin Secretion and Type 2 Diabetes
Current Molecular Medicine The Stress Rheostat: An Interplay Between the Unfolded Protein Response (UPR) and Autophagy in Neurodegeneration
Current Molecular Medicine The Genetic Basis of Kidney Cancer: Why is Tuberous Sclerosis Complex Often Overlooked?
Current Molecular Medicine Hypertension to Heart Failure: New Developmental Strategies do not Cross a Clinical and Therapeutic Divide
Current Pharmaceutical Design Age-Dependent Changes of the temporal Order - Causes and Treatment
Current Aging Science Porphyrin Photosensitised Processes in the Prevention and Treatment of Water- and Vector-Borne Diseases
Current Medicinal Chemistry High Throughput Screening Methodologies Classified for Major Drug Target Classes According to Target Signaling Pathways
Combinatorial Chemistry & High Throughput Screening Nonalcoholic Fatty Liver Disease (NAFLD) for Primary Care Providers: Beyond the Liver
Current Hypertension Reviews The Role of Coagulation and Fibrinolysis in the Pathogenesis of Acute Lung Injury
Current Respiratory Medicine Reviews Placebo Effect in Obsessive-Compulsive Disorder (OCD). Placebo Response and Placebo Responders in OCD: The Trend Over Time
Current Neuropharmacology Vitamin D, Sunlight and Cancer Connection
Anti-Cancer Agents in Medicinal Chemistry MicroRNA: Implications for Alzheimer Disease and other Human CNS Disorders
Current Genomics MicroRNA and Cancer: Tiny Molecules with Major Implications
Current Genomics Branched-Chain Amino Acids and Pigment Epithelium-Derived Factor: Novel Therapeutic Agents for Hepatitis C Virus-Associated Insulin Resistance
Current Medicinal Chemistry Monoclonal Antibodies: A New Era in the Treatment of Non-Hodgkins Lymphoma
Current Pharmaceutical Biotechnology Epigenetic Regulation and Therapeutic Approaches in Cancer
Current Topics in Medicinal Chemistry Occupational Exposure and Occupational Asthma: Difference in Practices in Developed Countries v/s Developing Countries
Current Respiratory Medicine Reviews