Abstract
A range of nanometer-sized materials such as metal nanoparticles, semiconductor quantum dots, metal oxide nanostructures, carbon nanomaterials and polymer nanoparticles, have been introduced into polymerase chain reactions (PCR). They have shown to remarkably improve PCR amplification in sensitivity, specificity and extension rate. In this review, we will try to describe the development of nanomaterials-based PCR, including the types of nanomaterials, optimization of properties and mechanisms. In particular, several mechanisms have been introduced in view of special physical and chemical properties of nanomaterials.
Keywords: Nanomaterial, metal nanoparticle, QDs, metal oxide nanostructure, carbon nanomaterial, polymer, polymerase, DNA, PCR, DNA detection, optimization, specificity, sensitivity, extend rate, yields, hot-start, mechanism, walled carbon nanotubes (SWCNTs), gold nanoparticles, Silver nanoparticles, CdSe QDs, ZnO, Carbon nanotubes(CNTs), Carbon nanoparticles (CNPs), C60, poly(N-vinylpyrrolidone), Dendrimer, GC-rich efficiency, Japanese encephalitis virus (JEV), mosquito-borne neurotropic flavivirus, RT-PCR, Semiconductor Nanoparticles, Semiconductor quantum dots, mercaptoacetic, streptavidin, Metal Oxide Nanomaterials, walled carbon nanotubes (MWCNTs), carbon nanopowder, quantitative real-time, Nanometer-size Polymers, amphiphilic poly(N-vinylpyrrolidone) (PVP) copolymer, Copolymer 9, GC-rich -actin, pUC18 DNA, poly (amidoamine) (PAMAM) dendrimers, NANOPCR, scanning electron microscopy (SEM), croscopy (HRTEM), dithiothreitol (DTT), X-ray photoelectron spectroscopy (XPS)
Call for Papers in Thematic Issues
Advances of Heterocyclic Chemistry with Pesticide Activity
Global food safety and security will continue to be a global concern for the next 50 years and beyond. Plant diseases have had a significant impact on food safety and security throughout the entire food chain, from primary production to consumption. While conventional chemical pesticides have been traditionally used for ...read more
Calculation design of covalent/metal organic framework based catalysts
This research area combines theoretical computation and screening with machine learning for the design of covalent/metal organic framework-based catalysts, bridging the disciplines of organic chemistry, physical chemistry, computational chemistry, materials science, and machine learning. It covers several critical aspects: designing and synthesizing organic catalysts for improved performance, applying computational methods ...read more
Carbohydrates conversion in biofuels and bioproducts
Biomass pretreatment, hydrolysis, and saccharification of carbohydrates, and sugars bioconversion in biofuels and bioproducts within a biorefinery framework. Carbohydrates derived from woody biomass, agricultural wastes, algae, sewage sludge, or any other lignocellulosic feedstock are included in this issue. Simulation, techno-economic analysis, and life cycle analysis of a biorefinery process are ...read more
Catalytic C-H bond activation as a tool for functionalization of heterocycles
The major topic is the functionalization of heterocycles through catalyzed C-H bond activation. The strategies based on C-H activation not only provide straightforward formation of C-C or C-X bonds but, more importantly, allow for the avoidance of pre-functionalization of one or two of the cross-coupling partners. The beneficial impact of ...read more
Related Books
Advances in Organic Synthesis
The Synthetic Methods, Structures, and Properties of the Ca-C σ Bond Organocalcium Containing Compounds
Advances in Organic Synthesis
Chemistry of Bipyrazoles: Synthesis and Applications
Advances in Organic Synthesis
Advanced Nanocatalysis for Organic Synthesis and Electroanalysis
Advances in Organic Synthesis
Advances in Organic Synthesis
17