Layer-by-Layer Polyelectrolyte Assembles Involving DNA as a Platform for DNA Sensors

Gennady      A. Evtugyn; Tibor      Hianik

Abstract

The development of DNA-sensors has become significantly important in the past decades due to prospects of application in medicine, biotechnology and exploring fundamental problems related to cell biology and DNA functioning. Layer-by-layer (LbL) immobilization provides unique approach to the implementation of DNA into the surface sensing layers, a crucial step of DNA-sensor development. The review considers main aspects of LbL assembling in DNA-sensor development and application for the detection of complementary oligonucleotides and DNA damage assessment. Besides, electrostatic assembling due to stepwise accumulation of oppositely charged layers, various combinations of covalent binding and affine immobilization are also considered. The characteristics of DNA containing multilayers onto the solid support and the effect of the immobilization techniques and layers assembled on the performance of appropriate DNAsensors are summarized for different target analytes.

Keywords: DNA-sensor, Biosensor, Layer-by-layer assembling, Self-assembled monolayers, Polyelectrolytes, DNA hybridization, DNA damaging factors, complementary oligonucleotides, affine immobilization, analytes, genetic hazard assessment, gene mapping, bridging metal cations, regulatory proteins, Langmuir-Blodgett (LB) films, oxymetallate layers, PAH, –, polyallylamine hydrochloride, PEI, polyethylene imine, PDDA, polydiallyldimethylammonium chloride, PVP, polyvinyl pyridine, PPy, polypyrrole, particle light scattering, ellipsometry, electronic microscopy, surface plasmon resonance (SPR), circular dichroism measurements, ferrocenyl moieties, polyamines, ferrocenecarboxyaldehyde, silane matrices, sulfonate substituents, thiolated DNA probes, carbodiimide, dendrimers, poly-L-lysine, Single-walled carbon nanotubes, magnetic ferrite nanoparticles, confocal microscopy, Non-covalent weak interactions, Affine interactions, Langmuir-Schaefer method, octadecylamine (ODA), dioctadecyldimethylamonium (DODA), sturgeon sperm, Electropolymerization, aniline, pyrrole, thiophene, phenothiazines, Piezoelectric Aptasensors, PNA Sensors