Abstract
Nucleic acids transfer has been steadily improving over the years and is slowly starting to fulfill its long awaited promises. In the beginning, viral approaches raised strong safety concerns that are now answered by various nonviral techniques. Among the physical approaches developed, nucleic acids electrotransfer is probably the one with the highest momentum. Here we review the present knowledge on the mechanistic and practical aspects of in vivo nucleic acids electrotransfer. For each step of this procedure we present different strategies that are used, with their advantages and drawbacks. As we report here, practical solutions have been found to overcome each limiting step in the procedure and to improve its outcome. Some crucial issues are beyond the application of the electric pulses itself, like the administration (i.e., in almost all of the cases, the injection) of the nucleic acids to the tissue or the body. High efficiency and safety are at reach if all the present knowledge and strategies are put to use. Electrotransfer is now a mature technique as proven by the fact that clinical trials using nucleic acids electrotransfer have already started within the past few years.
Keywords: DNA injection, electric pulses, electropermeabilization, electrophoresis, electroporation, minicircle, plasmid
Current Gene Therapy
Title: Nucleic Acids Electrotransfer In Vivo: Mechanisms and Practical Aspects
Volume: 10 Issue: 4
Author(s): Franck M. Andre and Lluis M. Mir
Affiliation:
Keywords: DNA injection, electric pulses, electropermeabilization, electrophoresis, electroporation, minicircle, plasmid
Abstract: Nucleic acids transfer has been steadily improving over the years and is slowly starting to fulfill its long awaited promises. In the beginning, viral approaches raised strong safety concerns that are now answered by various nonviral techniques. Among the physical approaches developed, nucleic acids electrotransfer is probably the one with the highest momentum. Here we review the present knowledge on the mechanistic and practical aspects of in vivo nucleic acids electrotransfer. For each step of this procedure we present different strategies that are used, with their advantages and drawbacks. As we report here, practical solutions have been found to overcome each limiting step in the procedure and to improve its outcome. Some crucial issues are beyond the application of the electric pulses itself, like the administration (i.e., in almost all of the cases, the injection) of the nucleic acids to the tissue or the body. High efficiency and safety are at reach if all the present knowledge and strategies are put to use. Electrotransfer is now a mature technique as proven by the fact that clinical trials using nucleic acids electrotransfer have already started within the past few years.
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Cite this article as:
M. Andre Franck and M. Mir Lluis, Nucleic Acids Electrotransfer In Vivo: Mechanisms and Practical Aspects, Current Gene Therapy 2010; 10 (4) . https://dx.doi.org/10.2174/156652310791823380
DOI https://dx.doi.org/10.2174/156652310791823380 |
Print ISSN 1566-5232 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5631 |
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