Abstract
Monoclonal antibodies capable of recognizing antigens with high affinity and specificity represent a wellestablished class of biological agents. Since the development of hybridoma technology in 1975, advances in recombinant DNA technologies and computational and biophysical methods have allowed us to develop a better understanding of the relationships between antibody sequence, structure, and function. These advances enable us to manipulate antibody sequences with the goal of improving upon, or creating new biological or biophysical properties. In this review we will focus on recent successes in using structure-guided computational methods to design antibodies and antibody-like molecules with optimized affinity and specificity to antigen and for enhancing protein stability.
Keywords: Antibody engineering, structure-based design, affinity maturation, effector function, protein stability
Current Computer-Aided Drug Design
Title: Structure-Guided Design of Antibodies
Volume: 6 Issue: 2
Author(s): Justin A. Caravella, Deping Wang, Scott M. Glaser and Alexey Lugovskoy
Affiliation:
Keywords: Antibody engineering, structure-based design, affinity maturation, effector function, protein stability
Abstract: Monoclonal antibodies capable of recognizing antigens with high affinity and specificity represent a wellestablished class of biological agents. Since the development of hybridoma technology in 1975, advances in recombinant DNA technologies and computational and biophysical methods have allowed us to develop a better understanding of the relationships between antibody sequence, structure, and function. These advances enable us to manipulate antibody sequences with the goal of improving upon, or creating new biological or biophysical properties. In this review we will focus on recent successes in using structure-guided computational methods to design antibodies and antibody-like molecules with optimized affinity and specificity to antigen and for enhancing protein stability.
Export Options
About this article
Cite this article as:
A. Caravella Justin, Wang Deping, M. Glaser Scott and Lugovskoy Alexey, Structure-Guided Design of Antibodies, Current Computer-Aided Drug Design 2010; 6 (2) . https://dx.doi.org/10.2174/157340910791202469
DOI https://dx.doi.org/10.2174/157340910791202469 |
Print ISSN 1573-4099 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6697 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Recent Patents Relating to Tumor Suppressor Genes
Recent Patents on DNA & Gene Sequences Diagnostic Methods in the Detection of Prostate Cancer: Prospective Observational Study
Current Medical Imaging The LIM Protein fhlA is Essential for Heart Chamber Development in Zebrafish Embryos
Current Molecular Medicine Stable Isotope Techniques to Develop and Monitor Nutrition Interventions
Current Nutrition & Food Science Network Pharmacology Strategies Toward Multi-Target Anticancer Therapies: From Computational Models to Experimental Design Principles
Current Pharmaceutical Design Perspectives in Nanomedicine-Based Research Towards Cancer Therapies
Current Nanoscience Flavonoids as Multi-Target Compounds in Drug Discovery
Mini-Reviews in Organic Chemistry Human Papillomavirus (HPV) Vaccines as an Option for Preventing Cervical Malignancies: (How) Effective and Safe?
Current Pharmaceutical Design Quantitative Analysis of Microbial Metabolism in the Human Large Intestine
Current Nutrition & Food Science Function and Regulation of Let-7 Family microRNAs
MicroRNA Astatine-211: Production and Availability
Current Radiopharmaceuticals Meet Our Editorial Board Member:
Current Medicinal Chemistry Metabotropic Glutamate Receptors in Central Nervous System Diseases
Current Drug Targets Recent Advances of Terahertz Imaging Technologies
Recent Patents on Signal Processing (Discontinued) Targeting the Spindle Assembly Checkpoint for Breast Cancer Treatment
Current Cancer Drug Targets Synthesis and In Vitro Biochemical Evaluation of a Series of Cycloalkyl Esters of 4-Sulfamoylated Benzoic Acid as Inhibitors of Estrone Sulfatase (ES)
Letters in Drug Design & Discovery Occurrence, Functions and Biological Significance of Arginine-Rich Proteins
Current Protein & Peptide Science The Transcriptome in Blood: Challenges and Solutions for Robust Expression Profiling
Current Molecular Medicine KRAB-Zinc Finger Proteins: A Repressor Family Displaying Multiple Biological Functions
Current Genomics The Neurotachykinin NK1 Receptor – A Novel Target for Diagnostics and Therapy
Current Molecular Imaging (Discontinued)