Abstract
Protein refolding is still on trial-and-error basis. Here we describe step-wise dialysis refolding, in which denaturant concentration is altered in step-wise fashion. This technology controls the folding pathway by adjusting the concentrations of the denaturant and other solvent additives to induce sequential folding or disulfide formation.
Keywords: Step-wise refolding, arginine, aggregation, antibody fragment, dialysis
Current Pharmaceutical Biotechnology
Title: Step-Wise Refolding of Recombinant Proteins
Volume: 11 Issue: 3
Author(s): Kouhei Tsumoto, Tsutomu Arakawa and Linda Chen
Affiliation:
Keywords: Step-wise refolding, arginine, aggregation, antibody fragment, dialysis
Abstract: Protein refolding is still on trial-and-error basis. Here we describe step-wise dialysis refolding, in which denaturant concentration is altered in step-wise fashion. This technology controls the folding pathway by adjusting the concentrations of the denaturant and other solvent additives to induce sequential folding or disulfide formation.
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Cite this article as:
Tsumoto Kouhei, Arakawa Tsutomu and Chen Linda, Step-Wise Refolding of Recombinant Proteins, Current Pharmaceutical Biotechnology 2010; 11 (3) . https://dx.doi.org/10.2174/138920110791111979
DOI https://dx.doi.org/10.2174/138920110791111979 |
Print ISSN 1389-2010 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4316 |
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