A Highly Controllable Reconstituted Cell-Free System -a Breakthrough in Protein Synthesis Research

Title: A Highly Controllable Reconstituted Cell-Free System -a Breakthrough in Protein Synthesis Research

Volume: 11 Issue: 3

Author(s): Hiroyuki Ohashi, Takashi Kanamori, Yoshihiro Shimizu and Takuya Ueda

Affiliation:

Keywords: Cell-free protein synthesis, in vitro translation, chaperone, disulfide bond formation, in vitro selection, unnatural amino acid

Abstract: The PURE system is a highly controllable cell-free protein synthesis system composed of individually prepared components that are required for protein synthesis in Escherichia coli. The PURE system contains neither nucleases nor proteases, both of which degrade DNA or mRNA templates and proteins. The protein products are easily purified using affinity chromatography to remove the tagged protein factors. The PURE system should help to create new fields in protein research.

Cite this article as:

Ohashi Hiroyuki, Kanamori Takashi, Shimizu Yoshihiro and Ueda Takuya, A Highly Controllable Reconstituted Cell-Free System -a Breakthrough in Protein Synthesis Research, Current Pharmaceutical Biotechnology 2010; 11 (3) . https://dx.doi.org/10.2174/138920110791111889