Abstract
Development and progression of breast cancer can be caused by increased estradiol activity, which stimulates cell proliferation. Inhibitors of type 1 17β-hydroxysteroid dehydrogenase (17β-HSD) enzyme inhibit estradiol biosynthesis and therefore have potential anticancer activity. In this study two new trans-cinnamic acid esters were established as inhibitors of the human recombinant type 1 17β-HSD enzyme. Studied compounds are poorly water soluble and have low stability in aqueous medium. Free inhibitors were tested on T-47D cells, which express the target enzyme, but did not exert any biological effect up to 100 μM. Therefore, novel poly(η- caprolactone) nanoparticles loaded with the inhibitors were formulated and their effects on T-47D cells were investigated. Prepared nanoparticles had regular spherical shape and mean diameters in the range of 130-170 nm, low polydispersity, high zeta potential and entrapment efficiency. Effective uptake of nanoparticles into T-47D breast cancer cells was confirmed, indicating the possibility to deliver incorporated inhibitor into the cell cytoplasm, where it is released and inhibits the target enzyme. Furthermore, the effect of delivered inhibitors resulted in reduced cell viability and changes in cell morphology. It is anticipated that engineering of efficient delivery system for new poorly soluble inhibitors led to a significant improvement of their biological activity and stability.
Keywords: Nanoparticles, enzyme inhibitors, T-47D cells, cellular uptake, drug delivery, breast cancer
Current Nanoscience
Title: Effect of Free and in Poly(η-caprolactone) Nanoparticles Incorporated New Type 1 17β -Hydroxysteroid Dehydrogenase Inhibitors on Cancer Cells
Volume: 6 Issue: 1
Author(s): Petra Kocbek, Karmen Teskac, Petra Brozic, Tea Lanisnik Rizner, Stanislav Gobec and Julijana Kristl
Affiliation:
Keywords: Nanoparticles, enzyme inhibitors, T-47D cells, cellular uptake, drug delivery, breast cancer
Abstract: Development and progression of breast cancer can be caused by increased estradiol activity, which stimulates cell proliferation. Inhibitors of type 1 17β-hydroxysteroid dehydrogenase (17β-HSD) enzyme inhibit estradiol biosynthesis and therefore have potential anticancer activity. In this study two new trans-cinnamic acid esters were established as inhibitors of the human recombinant type 1 17β-HSD enzyme. Studied compounds are poorly water soluble and have low stability in aqueous medium. Free inhibitors were tested on T-47D cells, which express the target enzyme, but did not exert any biological effect up to 100 μM. Therefore, novel poly(η- caprolactone) nanoparticles loaded with the inhibitors were formulated and their effects on T-47D cells were investigated. Prepared nanoparticles had regular spherical shape and mean diameters in the range of 130-170 nm, low polydispersity, high zeta potential and entrapment efficiency. Effective uptake of nanoparticles into T-47D breast cancer cells was confirmed, indicating the possibility to deliver incorporated inhibitor into the cell cytoplasm, where it is released and inhibits the target enzyme. Furthermore, the effect of delivered inhibitors resulted in reduced cell viability and changes in cell morphology. It is anticipated that engineering of efficient delivery system for new poorly soluble inhibitors led to a significant improvement of their biological activity and stability.
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Kocbek Petra, Teskac Karmen, Brozic Petra, Rizner Lanisnik Tea, Gobec Stanislav and Kristl Julijana, Effect of Free and in Poly(η-caprolactone) Nanoparticles Incorporated New Type 1 17β -Hydroxysteroid Dehydrogenase Inhibitors on Cancer Cells, Current Nanoscience 2010; 6 (1) . https://dx.doi.org/10.2174/157341310790226397
DOI https://dx.doi.org/10.2174/157341310790226397 |
Print ISSN 1573-4137 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6786 |
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