Abstract
Introduction: a current problem with the Human papillomavirus (HPV) genital infection is to detect HPV presence and activity in high risk women. Material and methods: 190 women at risk for HPV-infection underwent a Pap Test as well as a cervico-vaginal mucus sample analysis. The genome amplification of ORF L1 was by REAL-Time PCR by direct sequencing in capillary elettrophoresis of amplified product in Real Time (by ABI PRISM® 310 Genetic Analyzer, Applied Biosystem, USA), followed by HPV genotyping using oligonucleotide probe hybridization. Degenerate primers My09/11, with 450 bp product amplified were utilized in Real Time and in Direct Sequencing. Furthermore, samples were evaluated by mRNA-HPV test to detect the presence of E6 and E7 transcripts. The results were compared with cytology. Results: a total of 62 women were positive for HPV infection (32.6%) and 19 of these had one or more high-risk HPVs (30.6%); the concordance between the two assays was 78.9%, with 21.1% of totally or partially discordant results. Cytological results showed mRNA presence in 4 low grade and 2 high grade squamous intraepithelial lesions. Conclusion: the results suggest the potential of E6/E7 detection to target the presence of a transforming HPV infection.
Keywords: HPV, Human papillomavirus, high-risk HPV, cervical cancer, DNA, mRNA, NASBA, REAL-Time PCR squamous intraepithelial lesion, SIL, cervical intraepithelial neoplasia
Current Pharmaceutical Biotechnology
Title: HPV Viral Activity by mRNA-HPV Molecular Analysis to Screen the Transforming Infections in Precancer Cervical Lesions
Volume: 10 Issue: 8
Author(s): Andrea Tinelli, Giuseppe Leo, Maurizio Pisano, Fabio Storelli, Sara Leo, Daniele Vergara and Antonio Malvasi
Affiliation:
Keywords: HPV, Human papillomavirus, high-risk HPV, cervical cancer, DNA, mRNA, NASBA, REAL-Time PCR squamous intraepithelial lesion, SIL, cervical intraepithelial neoplasia
Abstract: Introduction: a current problem with the Human papillomavirus (HPV) genital infection is to detect HPV presence and activity in high risk women. Material and methods: 190 women at risk for HPV-infection underwent a Pap Test as well as a cervico-vaginal mucus sample analysis. The genome amplification of ORF L1 was by REAL-Time PCR by direct sequencing in capillary elettrophoresis of amplified product in Real Time (by ABI PRISM® 310 Genetic Analyzer, Applied Biosystem, USA), followed by HPV genotyping using oligonucleotide probe hybridization. Degenerate primers My09/11, with 450 bp product amplified were utilized in Real Time and in Direct Sequencing. Furthermore, samples were evaluated by mRNA-HPV test to detect the presence of E6 and E7 transcripts. The results were compared with cytology. Results: a total of 62 women were positive for HPV infection (32.6%) and 19 of these had one or more high-risk HPVs (30.6%); the concordance between the two assays was 78.9%, with 21.1% of totally or partially discordant results. Cytological results showed mRNA presence in 4 low grade and 2 high grade squamous intraepithelial lesions. Conclusion: the results suggest the potential of E6/E7 detection to target the presence of a transforming HPV infection.
Export Options
About this article
Cite this article as:
Tinelli Andrea, Leo Giuseppe, Pisano Maurizio, Storelli Fabio, Leo Sara, Vergara Daniele and Malvasi Antonio, HPV Viral Activity by mRNA-HPV Molecular Analysis to Screen the Transforming Infections in Precancer Cervical Lesions, Current Pharmaceutical Biotechnology 2009; 10 (8) . https://dx.doi.org/10.2174/138920109789978739
DOI https://dx.doi.org/10.2174/138920109789978739 |
Print ISSN 1389-2010 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4316 |
Call for Papers in Thematic Issues
Artificial Intelligence in Bioinformatics
Bioinformatics is an interdisciplinary field that analyzes and explores biological data. This field combines biology and information system. Artificial Intelligence (AI) has attracted great attention as it tries to replicate human intelligence. It has become common technology for analyzing and solving complex data and problems and encompasses sub-fields of machine ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Pharmacological Profile and Pharmacogenomics of Anti-Cancer Drugs Used for Targeted Therapy
Current Cancer Drug Targets Natural Polyphenols and their Synthetic Analogs as Emerging Anticancer Agents
Current Drug Targets Drug Development Strategy for Type 2 Diabetes: Targeting Positive Energy Balances
Current Drug Targets Nanocarriers Based Anticancer Drugs: Current Scenario and Future Perceptions
Current Drug Targets The Role of Culture and Ethnicity in the Adjustment to Gynecological Cancer
Current Women`s Health Reviews Cervical Cancer During Pregnancy – An Approach to Diagnosis and Management
Current Women`s Health Reviews The Role of Connexins in Carcinogenesis: Review of Current Knowledge
Current Signal Transduction Therapy Recent Advances in Carbon Nanotubes as Delivery Systems for Anticancer Drugs
Current Medicinal Chemistry Aldo-Keto Reductase Family 1 Member B1 Inhibitors: Old Drugs with New Perspectives
Recent Patents on Anti-Cancer Drug Discovery Prognostic and Therapeutic Implications of MicroRNA in Malignant Pleural Mesothelioma
MicroRNA Advances of Metal Enhanced Fluorescence Applications for the Biomedical Field
Current Proteomics Cytotoxic and Allergenic Potential of Bioactive Proteins and Peptides
Current Pharmaceutical Design Translation of a Tissue-Selective Rexinoid, UAB30, to the Clinic for Breast Cancer Prevention
Current Topics in Medicinal Chemistry Prodrugs for Targeted Tumor Therapies: Recent Developments in ADEPT, GDEPT and PMT
Current Pharmaceutical Design Carbohydrate Antigens: Synthesis Aspects and Immunological Applications in Cancer
Mini-Reviews in Medicinal Chemistry Therapeutic Antibodies for Spinal Cord Injury
CNS & Neurological Disorders - Drug Targets Imaging of Hypoxia Using PET and MRI
Current Pharmaceutical Biotechnology Inhibiting TLR9 Signaling Stimulates Apoptosis and Cell Cycle Arrest, and Alleviates Angiogenic Property in Human Cervical Cancer Cells
Endocrine, Metabolic & Immune Disorders - Drug Targets Potential Prophylactic and Therapeutic Vaccines for HSV Infections
Current Pharmaceutical Design Targeting Hypoxia for Sensitization of Tumors to Radio- and Chemotherapy
Current Cancer Drug Targets