Mass Spectrometry in High Throughput Screening: A Case Study on Acetyl-Coenzyme A Carboxylase using RapidFire® – Mass Spectrometry (RF-MS)

Title: Mass Spectrometry in High Throughput Screening: A Case Study on Acetyl-Coenzyme A Carboxylase using RapidFire® – Mass Spectrometry (RF-MS)

Volume: 12 Issue: 8

Author(s): Maxine Jonas, William A. LaMarr and Can Ozbal

Affiliation:

Keywords: High throughput screening, acetyl-coenzyme A carboxylase, RapidFire, mass spectrometry

Abstract: In this review various technologies and approaches for the utilization of mass spectrometry in high throughput analyses are discussed. The use of quadrupole-based mass spectrometry in the screening of chemical libraries against enzymatic targets for the identification of inhibitors and/or activators is highlighted. The RapidFire mass spectrometry system, an integrated on-line solid-phase extraction system interfaced to a triple-quadrupole mass spectrometer is described in detail, and the identification of a series of inhibitors of the acetyl-coenzyme A carboxylase (ACC) assay is described.

Cite this article as:

Jonas Maxine, LaMarr A. William and Ozbal Can, Mass Spectrometry in High Throughput Screening: A Case Study on Acetyl-Coenzyme A Carboxylase using RapidFire® – Mass Spectrometry (RF-MS), Combinatorial Chemistry & High Throughput Screening 2009; 12 (8) . https://dx.doi.org/10.2174/138620709789104924