High Throughput Screening of Gene Functions in Mammalian Cells Using Reversely Transfected Cell Arrays: Review And Protocol

Title: High Throughput Screening of Gene Functions in Mammalian Cells Using Reversely Transfected Cell Arrays: Review And Protocol

Volume: 11 Issue: 2

Author(s): Michael Sturzl*, Andreas Konrad, Gaby Sander, Effi Wies, Frank Neipel, Elisabeth Naschberger, Simone Reipschlager, Nathalie Gonin-Laurent, Raymund E. Horch, Ulrich Kneser, Werner Hohenberger, Holger Erfle and Mathias Thurau

Affiliation:

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Keywords: Reverse transfection, cell microarray, cell chip, apoptosis, proliferation, siRNA

Abstract: Reversely transfected cell microarrays (RTCM) have been introduced as a method for parallel high throughput analysis of gene functions in mammalian cells. Hundreds to thousands of different recombinant DNA or RNA molecules can be transfected into different cell clusters at the same time on a single glass slide with this method. This allows either the simultaneous overexpression or - by using the recently developed RNA interference (RNAi) techniques - knockdown of a huge number of target genes. A growing number of sophisticated detection systems have been established to determine quantitatively the effects of the transfected molecules on the cell phenotype. Several different cell types have been successfully used for this procedure. This review summarizes the presently available knowledge on this technique and provides a laboratory protocol.

Cite this article as:

Sturzl Michael*, Konrad Andreas, Sander Gaby, Wies Effi, Neipel Frank, Naschberger Elisabeth, Reipschlager Simone, Gonin-Laurent Nathalie, Horch E. Raymund, Kneser Ulrich, Hohenberger Werner, Erfle Holger and Thurau Mathias, High Throughput Screening of Gene Functions in Mammalian Cells Using Reversely Transfected Cell Arrays: Review And Protocol, Combinatorial Chemistry & High Throughput Screening 2008; 11 (2) . https://dx.doi.org/10.2174/138620708783744499