Abstract
Cells utilize myo-inositol for osmoregulation and phosphatidylinositol signaling. Mass spectrometric and in vivo magnetic resonance spectroscopic techniques have been complementarily used in our laboratories to investigate brain myo-inositol metabolism. Mass spectrometric quantitation methods are surveyed focusing primarily on derivatization reactions, gas chromatographic separation and detection of ions. Monitoring of the m/z 373 fragment ion generated from acetate derivative provides precise quantitation of myo-inositol in biological matrices. The technique and its clinical applications are discussed. Measurement of myo-inositol transport using a stable isotope technique is illustrated for cultured neurons. In addition, the possible use of the technique in probing phosphatidylinositol turnover is discussed. An in vivo 1H magnetic resonance spectroscopic technique is described for measuring the absolute concentration of myo-inositol in human brain. Magnetic resonance spectroscopy with short echo-time enables detection of the resonance peak of myo-inositol (3.56 ppm) when the water resonance peak is suppressed by narrow band radio-frequency pulses. The review focuses on an external reference method involving collection of data from the human subject and the phantom containing aqueous myo-inositol standard solution in the same scanning session. The method takes into account differences in longitudinal and transverse relaxation time constants of myo-inositol between brain tissue and aqueous solution. Application of the technique is illustrated by measuring brain myo-inositol in Down syndrome adults and Alzheimer disease patients. Advantages and limitations of this noninvasive technique in monitoring metabolic processes are discussed.
Current Organic Chemistry
Title: Measurement of myo-Inositol in Biological Systems by Mass Spectrometric and In Vivo 1H Magnetic Resonance Spectroscopic Techniques
Volume: 4 Issue: 1
Author(s): H. Umesha Shetty and Wei Huang
Affiliation:
Abstract: Cells utilize myo-inositol for osmoregulation and phosphatidylinositol signaling. Mass spectrometric and in vivo magnetic resonance spectroscopic techniques have been complementarily used in our laboratories to investigate brain myo-inositol metabolism. Mass spectrometric quantitation methods are surveyed focusing primarily on derivatization reactions, gas chromatographic separation and detection of ions. Monitoring of the m/z 373 fragment ion generated from acetate derivative provides precise quantitation of myo-inositol in biological matrices. The technique and its clinical applications are discussed. Measurement of myo-inositol transport using a stable isotope technique is illustrated for cultured neurons. In addition, the possible use of the technique in probing phosphatidylinositol turnover is discussed. An in vivo 1H magnetic resonance spectroscopic technique is described for measuring the absolute concentration of myo-inositol in human brain. Magnetic resonance spectroscopy with short echo-time enables detection of the resonance peak of myo-inositol (3.56 ppm) when the water resonance peak is suppressed by narrow band radio-frequency pulses. The review focuses on an external reference method involving collection of data from the human subject and the phantom containing aqueous myo-inositol standard solution in the same scanning session. The method takes into account differences in longitudinal and transverse relaxation time constants of myo-inositol between brain tissue and aqueous solution. Application of the technique is illustrated by measuring brain myo-inositol in Down syndrome adults and Alzheimer disease patients. Advantages and limitations of this noninvasive technique in monitoring metabolic processes are discussed.
Export Options
About this article
Cite this article as:
Umesha Shetty H. and Huang Wei, Measurement of myo-Inositol in Biological Systems by Mass Spectrometric and In Vivo 1H Magnetic Resonance Spectroscopic Techniques, Current Organic Chemistry 2000; 4 (1) . https://dx.doi.org/10.2174/1385272003376382
DOI https://dx.doi.org/10.2174/1385272003376382 |
Print ISSN 1385-2728 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5348 |
Call for Papers in Thematic Issues
Advances of Heterocyclic Chemistry with Pesticide Activity
Global food safety and security will continue to be a global concern for the next 50 years and beyond. Plant diseases have had a significant impact on food safety and security throughout the entire food chain, from primary production to consumption. While conventional chemical pesticides have been traditionally used for ...read more
Calculation design of covalent/metal organic framework based catalysts
This research area combines theoretical computation and screening with machine learning for the design of covalent/metal organic framework-based catalysts, bridging the disciplines of organic chemistry, physical chemistry, computational chemistry, materials science, and machine learning. It covers several critical aspects: designing and synthesizing organic catalysts for improved performance, applying computational methods ...read more
Carbohydrates conversion in biofuels and bioproducts
Biomass pretreatment, hydrolysis, and saccharification of carbohydrates, and sugars bioconversion in biofuels and bioproducts within a biorefinery framework. Carbohydrates derived from woody biomass, agricultural wastes, algae, sewage sludge, or any other lignocellulosic feedstock are included in this issue. Simulation, techno-economic analysis, and life cycle analysis of a biorefinery process are ...read more
Catalytic C-H bond activation as a tool for functionalization of heterocycles
The major topic is the functionalization of heterocycles through catalyzed C-H bond activation. The strategies based on C-H activation not only provide straightforward formation of C-C or C-X bonds but, more importantly, allow for the avoidance of pre-functionalization of one or two of the cross-coupling partners. The beneficial impact of ...read more
Related Journals
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Fluoxetine Protects against Dendritic Spine Loss in Middle-aged APPswe/PSEN1dE9 Double Transgenic Alzheimer’s Disease Mice
Current Alzheimer Research Design, Synthesis and hMAO Inhibitory Screening of Novel 2-Pyrazoline Analogues
Combinatorial Chemistry & High Throughput Screening Chemoprevention of Colorectal Cancer: Progress or Pipedream?
Current Cancer Therapy Reviews Can Lipoprotein-associated Phospholipase A<sub>2</sub> be Used as a Predictor of Long-term Outcome in Patients with Acute Coronary Syndrome?
Current Cardiology Reviews Rational Basis for Nutraceuticals in the Treatment of Glaucoma
Current Neuropharmacology Approaches to Stabilize Amorphous Form - A Review
Current Drug Therapy Sialic Acid Recognition of the Pandemic Influenza 2009 H1N1 Virus: Binding Mechanism Between Human Receptor and Influenza Hemagglutinin
Protein & Peptide Letters Safety and Efficacy Assessment of a Sanitary Pad Containing Potassium Alum in Comparison to Ordinary Pads: A Cross-Over Trial
Current Women`s Health Reviews Methods for Identifying Cardiovascular Agents: A Review
Recent Patents on Cardiovascular Drug Discovery Toxic Effects of Palladium Compounds on the Isolated Rat Heart
Medicinal Chemistry An Overview on the Role of α -Synuclein in Experimental Models of Parkinson’s Disease from Pathogenesis to Therapeutics
CNS & Neurological Disorders - Drug Targets NS5A - From Obscurity to New Target for HCV Therapy
Recent Patents on Anti-Infective Drug Discovery The Food-gut Human Axis: The Effects of Diet on Gut Microbiota and Metabolome
Current Medicinal Chemistry New Insight into P-Glycoprotein as a Drug Target
Anti-Cancer Agents in Medicinal Chemistry Upregulation of Connexin-43 is Critical for Irradiation-induced Neuroinflammation
CNS & Neurological Disorders - Drug Targets New Developments in MALDI Imaging for Pathology Proteomic Studies
Current Pharmaceutical Design Chronic Myelogenous Leukemia
Current Cancer Therapy Reviews The Potential of Nano-Vehicle Mediated Therapy in Vasculitis and Multiple Sclerosis
Current Pharmaceutical Design A Review on Electrospun Nanofibers-based Electrochemical Sensor
Current Nanoscience Patent Selections
Recent Patents on DNA & Gene Sequences